4 results about "Bacterial colony" patented technology

The invention relates to an automatic calibration method of a bacterial colony selector. According to the invention, the bacterial colony selector serves as an experimental platform, a camera calibration system suitable for the bacterial colony selector is established, and transformation between coordinates of a bacterial colony image and coordinates of a 3D space is realized. On the basis of a Tsai two-step method, the improved camera calibration method is provided to overcome defects that the realization manner is complex and manual interaction operation is needed in a traditional camera calibration method, image processing technology is combined with camera calibration technology, and camera calibration is made automatic. Advantages of fewer iteration parameters, high solution speed and high precision of the Tsai two-step method are reserved. Thus, the method can be applied to a camera calibration system of the bacterial colony selector effectively, it is ensured that the bacterial colony selector can read coordinate information of the 3D space of a target bacterial colony accurately, and experimental data is used to verify the accuracy of calibration.

The invention discloses a preparation and identification method of taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine. The preparation and identification method is characterized by comprising the following steps: (1) amplification and identification of plasmid pMV261; (2) construction and identification of recombinant plasmid pMV261-TSOL18; and (3) construction and identification of the taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine, namely 1) preparation of BCG competent cells, 2) electrotransformation of the BCG competent cells by use of the recombinant plasmid pMV261-TSOL18, 3) identification of the taenia solium TSOL18 gene recombinant bacillus calmette guerin vaccine, namely picking up individual bacterial colony in the positive colonies to an M7H9ADC liquid culture medium containing Kana for shaking culture, taking the bacterial liquid for centrifuging; abandoning the liquid supernatant, washing by use of ddH2O, centrifuging and abandoning the liquid supernatant, adding ddH2O, and re-suspending before boiling water bath, putting in ice bath, centrifuging, and absorbing the liquid supernatant as a PCR template for PCR identification.

The invention discloses a pressing type anti-slip visible easy-to-press square counting surface sampling culture vessel. The culture vessel comprises a vessel bottom and a vessel cover, wherein the vessel bottom is provided with a first clamp buckle and a second clamp buckle which are of stable embedding structures; when being stressed, the embedding structures can be disengaged, so that the bottom side wall of a movable vessel and the first vessel bottom side wall generate relative displacement; when a plurality of culture vessels are put in a stacked way, a first bulge and a second bulge canbe used for limiting the relative movement of two adjacent culture vessels; through an observation window, the observation of tiny bacterial colonies can be more convenient; through a press table, the moving of the bottom side wall of the movable vessel can be more convenient; through square lines, the counting process of bacterial colonies can be simplified.