3 results about "Cell system" patented technology

The invention discloses a heat dissipation and thermal runway dispersion protection structure in a cell system. The structure comprises a module shell and at least one composite heat-conducting plate, wherein a plurality of unit cells are arranged in the module shell; and the composite heat-conducting plate is positioned in the module shell, is contacted with the module shell, is arranged between at least two unit cells to serve as a transfer medium of heat between a cell and the shell and control the heat transfer between the cells, and has a multilayer anisotropic heat-conducting structure which consists of at least one heat-conducting layer and at least one heat-insulating layer.

The invention discloses an integrated cell, an integrated cell stack including the integrated cell and an integrated cell system. The integrated cell comprises an electrolytic cell for generating hydrogen and oxygen, and a fuel cell for generating electricity by the hydrogen and oxygen, wherein the fuel cell and the electrolytic cell share a collector plate and receive the hydrogen and oxygen generated by the electrolytic cell. According to the invention, the electrolytic cell and the fuel cell are integrally arranged to form the integrated cell; the number of the collector plates used is reduced; the cost is greatly saved; the integration is higher after integral arrangement; the management and maintenance of the integrated cell are facilitated; the reliability is enhanced in the process of conveying the hydrogen generated by the electrolytic cell to the fuel cell to generate electricity by the fuel cell at short range; a complicated process of purifying the air when the air is utilized is avoided by utilization of the oxygen, and output voltage of the cell is further improved by utilizing pure oxygen as reaction gas.

The invention belongs to the technical field of biological preparation and in particular relates to an RXRalpha (Homo Sapiens Retinoid X Receptor alpha) gene knockout cell system with stable and low expression of an RXRalpha protein and a preparation method of the RXRalpha gene knockout cell system. The cell system is an RXRalpha gene knockout cell system; the knockout base number is an integral multiple of a number except 3; RXRalpha protein expression in a cell is remarkably reduced, the genotype is stable and can be passed to next generations, the stability of the RXRalpha low-expression cell system is greatly improved, and stable generation passage and continuous culture of the cell system can be facilitated. The invention provides the preparation method of the cell system. The preparation method comprises the following steps: according to RXRalpha gene sequence information, designing a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) knockout gRNA (Guide Ribonucleic Acid) sequence, establishing a gRNA expression vector, and carrying out in-vitro cell level detection on gRNA shearing activity; by using a nuclear transferring method, carrying out cotransfectionon an immortalized cell of human neuroblastoma, that is, an SK-N-SH cell, by using cas9 and the gRNA expression vector, carrying out G418 medicine screening so as to obtain stable cell cloning, carrying out a PCR (Polymerase Chain Reaction) and gene sequencing to identify that the cell is cell cloning of which the RXRalpha gene is knocked out for an integral multiple of a number except 3, therebyobtaining the RXRalpha gene knockout cell system with stable and low expression of the RXRalpha protein.