The invention discloses a kit for detecting HSV-2
nucleic acid, comprising a specific primer pair HSV2-F and HSV2-R for detecting
herpes simplex virus type 2, a fluorescent probe HSV2-P and a positivequality control substance. Both ends of the probe are modified separately, the 5' end is labeled with report
fluorescein or fluorescent dye, and the 3' end is labeled with any
minor groove binder without a fluorescent background. The positive
quality control substance is a solution of a recombinant PUC57
plasmid containing a
herpes simplex virus type 2 HSV-2
target gene gG sequence. The kit of the invention has the characteristics of fast and accurate detection,
high resolution, high specificity, high efficiency and low cost, can realize qualitative detection of
herpes simplex virus type 2 HSV-2, and has wide clinical application values.