5 results about "Fusarium oxysporum" patented technology

The invention discloses bacillus subtilis and an application thereof. The accession number of the bacillus subtilis BLG010 in the China General Microbiological Culture Collection Center is CGMCC No.5953. The bacillus subtilis BLG010 with number being CGMCC No.5953 can effectively inhibit the growth of fusarium oxysporum f. sp. cubense at low concentration and has great significance on control of banana wilt.

The invention discloses a quantitative detection method for FOC (Fusarium oxysporum f. sp. cubense (E.F. Smith) Snyd. & Hans.) race 4 from the soil. The method comprises the following steps: pretreating a sample to be detected and extracting DNA of the sample to be detected; preparing specific primers; conducting fluorescent nucleic acid isothermal amplification detection technique RealAmp reaction; constructing a RealAmp standard curve; and determining the result. The real-time fluorescent nucleic acid isothermal amplification detection technology provided by the invention is a novel nucleic acid detection technology combining a new generation of isothermal nucleic acid amplification technology with the real-time fluorescent detection technology, and has the advantages of high sensitivity, high specificity, low pollution and stable reaction. RealAmp can quantitatively detect pathogens; because of the characteristics of LAMP, Bst DNA polymerase has chain exchange capacity and good tolerance of inhibiting substances contained in the soil DNA extracts; the method carries out detection work on the soil, avoids cover opening and influence by contaminants and conducts on-site soil sample detection; the method for analysis and determination of reaction products is very simple; therefore, the method is suitable for wide application.

The invention discloses composition for reductive soil sterilization and an application of the composition. The composition comprises a cellulose degradation compound microbial agent formed by flavobacterium johnsoniae and pseudomonas fluorescens and further comprises corn stalk. The composition can change the microflora structure of soil and promote the corn stalk to degrade in the anaerobic environment to produce ammonia, methane, organic acid, hydrogen sulfide and other substances which have poisonous effects on fusarium oxysporum. By means of the composition, 99.9% of fusarium oxysporum can be killed, and 97.0% of pathogenic bacteria can still be killed in the dry period. Besides, the composition can notably improve the physical and chemical properties of soil, can effectively relievesecondary salinization of the soil, further can be used for controlling tomato wilt and has an improvement effect.

The invention provides a talaromyces pinophilus NJAU-L8 for preventing and controlling continuous crop rotation soil-borne wilt disease and use thereof. The strain has a preservation number of CGMCC No. 21476. The strain is used for reducing number of pathogens in watermelon and banana fusarium wilt planting soil, promoting plant growth and reducing morbidity. It is proved that the strain NJAU-L8 separated from rhizosphere of crop-rotation pineapple can effectively reduce colonization of fusarium oxysporum in rhizosphere of various crops and shows that the strain NJAU-L8 has potential of preventing and controlling wilt disease in actual production.

The invention discloses a panax notoginseng reverse osmosis protein gene PnOLP1. The gene PnOLP1 has a nucleotide sequence shown as SEQ ID NO:1, and encodes protein having an amino acid sequence shownas SEQ ID NO:2. It is verified by functional genomics related technical research that the gene PnOLP1 has the function of improving the antifungal property of plants; after the antifungal gene PnOLP1is constructed onto a plant expression vector and transferred into nicotiana tabacum for overexpression, the transgenic nicotiana tabacum plant has quite high in-vitro antifungal activity as a result, and the experimental result shows that the transgenic nicotiana tabacum overexpressing PnOLP1 has a significant inhibiting effect on the growth of four fungi including colletotrichum gloeosporioides, fusarium solani, fusarium oxysporum and nigrospora oryzae.