The invention provides an anguilla japonica fry fishing device. The anguilla japonica fry fishing device comprises fixed piles and collecting net arranged among the fixed piles, the front net opening of the collecting net is big, the rear net opening of the collecting net is small, the collecting wire is in a conical cylinder shape, the lower ends of the fixed piles are vertically arranged in mud of an anguilla japonica fishing point in an inserted mode, the front net opening of the collecting net is provided with a thick nylon rope circle to set the shape of the front net opening, the upper end of the fixed pile is connected with the thick nylon rope circle, and the rear net opening of the collecting net is provided with a tightening rope used for timely closing up the rear net opening; the collecting net comprises three sections of net bodies with different mesh sizes connected in sequence, and in addition, the collecting net is further connected with a floating apparatus. According to a using method of the anguilla japonica fry fishing device, the problem is solved that due to the adoption of a uniform size, the wild anguilla japonica fry resources are badly destroyed; meanwhile, the mortality and the physical labor intensity of fishermen in the anguilla japonica fry collecting process are lowered; the anguilla japonica fry fishing device is simple in structure and low in cost, and can be applied to different fishing positions.