The invention discloses a preparation method of a calcium sulfate whisker floor tile. The method comprises the following process step of preparing an ingredient which is prepared from the following raw materials in parts by weight: 120-140 parts of plastic particles, 11-13 parts of silica gel, 32-35 parts of a plasticizer, 1.4-1.6 parts of a dispersing agent, 9-10 parts of high-strength gypsum, 10-12 parts of titanium dioxide, 2-4 parts of calcium sulfate whiskers, 3-4 parts of dibasic lead stearate, 2.5-4 parts of a coupling reagent titanate, 4-6 parts of kieselguhr and 0.4-0.8 part of a pigment. The calcium sulfate whisker floor tile has an excellent antistatic effect, is free of lead, low in volatility, free of an oil paint or decoration, safe and reliable in a using process, good in wear resistance, moisture-proof, mothproof, corrosion-resistant, light in weight, low in price, waterproof and skidproof, and is more astringent in water, non-slip, good in heat conduction and heat retention properties, uniform in heat emission and heat-resistant.