4 results about "Single-nucleotide polymorphism" patented technology

The invention discloses a method for breeding Ili horses by detecting the SNP (single nucleotide polymorphism) of the Ili horses, and a kit employed by the method. Through the adoption of the method and the kit, the body size traits, namely body height, body length, chest girth and cannon circumference, of new groups for Ili horse meat can be remarkably enhanced, and the breeding of the new groups for Ili horse meat is promoted.

The invention discloses a method and a device for detecting a target gene or an SNP (Single Nucleotide Polymorphism) site of the target gene by combining asymmetric PCR (Polymerase Chain Reaction) with a magnetic sensitive detector. The method for detecting the gene marker by combining the asymmetric PCR with the magnetic sensitive detector comprises the following steps: (1) providing a biological sample and treating the biological sample; (2) carrying out asymmetric PCR (Polymerase Chain Reaction) amplification to obtain a single-chain gene marker with a biotin label; (3) hybridizing a single-chain gene marker, and combining the single-chain gene marker with the modified magnetic beads; and (4) detecting a signal through a magnetic sensitive detector. According to the method provided by the invention, the target gene can be directly amplified from the blood, special DNA extraction and purification are not needed, and the reaction time for obtaining the target gene from the blood can be shortened. The asymmetric PCR system provided by the invention can simultaneously amplify and detect a plurality of target genes, the target genes are single-stranded DNA with markers, the influence of complementary chains on the hybridization reaction of the capture probe is reduced, the single-stranded DNA is easy to hybridize and combine with the capture probe, and the intensity of an output signal is improved.