Microbiological crude oil decalcifying agent as well as preparation method and decalcifying method thereof

[0023] Take 245g of oilfield production fluid, and the total bacterial count reached 1.50×10 6 pieces / ml. Add 2% waste molasses, peptone 3000μg / g, 1.5g ammonium nitrate, 4g disodium hydrogen phosphate, 2g potassium dihydrogen phosphate, 0.5g magnesium sulfate micronutrients, place in a 250ml narrow mouth bottle and mix evenly, place in 40 Leave to ferment in an incubator at ℃ for 48 hours. The total acid content of the fermentation liquid is 0.33g / 100g measured by GB / T12717. Take it out and mix it with 130g of thick oil with a calcium content of 230μg / g. Add OP-10 emulsifier 50μg / g g, mixed on a shaker for 48 hours, the speed of the shaker is 120 rpm, the mixing temperature is 40°C, take it out, put the crude oil emulsion into the SH-2 electric desalination tester, add 25μg / g demulsifier KXDR and 25μg / g Demulsifier KXDH, under the conditions of oil-water separation temperature of 80°C, weak electric field of 500V / cm, residence time of weak electric field of 30

[0025] Take 97.5g of oilfield production fluid, and the total number of bacteria detected by microscope is 2.20×10 6pieces / ml. Add 6% waste molasses, peptone 5000 μg / g and 1g ammonium sulfate, 1.5g disodium hydrogen phosphate, 1g potassium dihydrogen phosphate, 0.2g magnesium sulfate micronutrients, place in a 250ml narrow-mouth bottle and mix evenly, place in 30 Leave to ferment in an incubator at ℃ for 24 hours, measure the total acid content of the fermentation broth with GB / T12717 as 0.25g / 100g, take it out and mix it with 130g of thick oil with a calcium content of 214μg / g, add OP-10 emulsifier 35μg / g Mix with AU-9 emulsifier 35μg / g, shaker for 24 hours, shaker speed 85 rpm, mixing temperature 30°C, take it out, put the crude oil emulsion into SH-2 electric desalination tester, add demulsifier KXDR70μg / g, dehydration under the conditions of oil-water separation temperature 110℃, weak electric field 500V / cm, residence time of weak electric field 20 minutes,

[0027] Take 390g of oilfield production fluid, the total number of bacteria detected by microscope is 3.90×10 6 pieces / ml. Add 4% waste molasses, peptone 7000 μg / g, 1.25g ammonium nitrate, 2.5g disodium hydrogen phosphate, 1.5g potassium dihydrogen phosphate, 0.3g magnesium sulfate micronutrients, place in a 250ml narrow mouth bottle and mix evenly, put Leave it to ferment in a 60°C incubator for 48 hours, measure the total acid content of the fermentation broth with GB / T12717 to 0.27g / 100g, take it out and mix it with 130g of Sudan oil with a calcium content of 702μg / g, add AU-9 emulsifier 100μg / g, mixing on a shaker for 48 hours, the rotating speed of the shaker is 140 rpm, the mixing temperature is 60°C, take it out, put the crude oil emulsion into the SH-2 electric desalination tester, add demulsifier KXDH 100μg / g, and separate the oil and water Dehydration under the conditions of temperature 130°C, weak electric field 500V / cm, weak electric field residence