Enzymatic hydrolysis lactoprotein zinc complex compound and preparation thereof

[0024] Casein was from Northwest Biotechnology Company; alkaline protease was purchased from Novozymes Biological Co., Ltd.

[0025] Take 0.6kg of casein, make it into a 6% (w / v) protein solution, add 0.6 grams of alkaline protease, adjust the enzymolysis temperature to 42°C, enzymolysis time is 6h, and the pH value is 8.0 for enzymolysis. After the end of the enzymolysis, Inactivate the enzyme at 90°C for 10 minutes, and perform ultrafiltration with an ultrafiltration device (organic ultrafiltration membrane with a molecular weight cut-off of 10 kDa) to obtain a relatively pure milk protein hydrolyzate, which is cooled to room temperature for later use.

[0026] The enzymatic hydrolyzed milk protein solution, according to the bioactive peptide and ZnSO 4 (in Zn 2+ The mass ratio of calculated) is 4:1, the reaction pH value is 6.0, the reaction temperature is 45° C., and the complexation time is 30 minutes for the complexation reaction to obtain the milk protein bioactive pept

[0028] Crude cheese: from Xinjiang; casein from Northwest Biotechnology Company; alkaline protease from Novozymes Biological Co., Ltd.

[0029] Take 10 kg of crude cheese and 0.5 kg of casein, make it into a 3% (w / v) protein solution, add 5 grams of alkaline protease, adjust the enzymolysis temperature to 425°C, enzymolysis time 10h, and pH value 7.0 for enzymolysis, After the enzymatic hydrolysis, the enzyme was inactivated at 60°C for 30 minutes, ultrafiltration was performed with an ultrafiltration device (ceramic ultrafiltration membrane with a molecular weight cut-off of 10 kDa), cooled to room temperature, and freeze-dried for later use.

[0030] Under certain environmental conditions, take the dry powder of enzymatically hydrolyzed milk protein and prepare a solution with a concentration of 7%. 2 (in Zn 2+ Calculation) mass ratio is 1:1, the reaction pH value is 4.0, the reaction temperature is 55°C, and the complexation time is 20min for the complexation reaction to ob

[0032] Casein: from Northwest Biotechnology Company; whey protein was purchased from Beijing Yinhe Road Company; alkaline protease was purchased from Beijing Fangshan Enzyme Preparation Factory.

[0033] Take 1.0kg of casein and 0.5kg of whey protein to prepare an 8% (w / v) protein solution, add 20 grams of alkaline protease, adjust the enzymolysis temperature to 50°C, enzymolysis time for 8 hours, and pH value 9.0 for enzymolysis After the enzymatic hydrolysis, inactivate the enzyme at 90°C for 10 minutes, cool to room temperature, and set aside.

[0034] The whole milk protein hydrolyzate was mixed with zinc citrate (as Zn 2+ Calculation) mass ratio was 1:5, reaction pH value was 9.0, reaction temperature was 35°C, and complexation time was 50 min for complexation reaction to obtain milk protein bioactive peptide zinc complex.