Dehydrogenase electrode, and preparation method and application thereof

[0023] Example 1. Preparation of polyaniline

[0024] Dissolve 186mg aniline monomer and 456mg potassium peroxodisulfate in 10mL 0.5M hydrochloric acid solution, react for 5 hours in an ice bath at 0~5℃, filter the precipitate, wash with hydrochloric acid and double distilled water respectively, and vacuum dry to obtain green poly Aniline (number average molecular weight 75400).

[0025] Example 2. Preparation of polyaniline

[0026] Add 294mg of aniline monomer and 348mg of manganese dioxide to 20mL of 1M hydrochloric acid solution, react in an ice bath at 0~10℃ for 10 hours, filter the precipitate, wash with hydrochloric acid and double distilled water, and vacuum dry to obtain green polyaniline ( The number average molecular weight is 32100).

[0027] Example 3. Polyaniline / carbon nanotube composite electrode

[0028] 1.5 mg of polyaniline (number average molecular weight 75400) prepared in Example 1 and 3 mg of carbon nanotubes (Shenzhen Nanoport Co., Ltd., diameter 2 , The ultrasonic frequency is 40kHz) for 1 hour, form a composite suspension of the two, take 6μL and apply it on a 0.07 cm2 glassy carbon substrate electrode, and dry at room temperature for 30 minutes to obtain a polyaniline / carbon nanotube composite electrode .

[0029] At the same time, the catalytic effect of the electrode on nicotinamide adenine dinucleotide (NADH, reduced type) was tested. The solution used in the test was a phosphate buffer solution pH=7. The results are shown in figure 2 . figure 2 The dotted line and the solid line are the cyclic voltammetry curves of the polyaniline / carbon nanotube composite electrode without and containing 4mM nicotinamide adenine dinucleotide (NADH, reduced type), and the potential scanning speed is 20mVs.