Immune colloidal gold test strip for simultaneously carrying out residue detection and analysis on Tylosin and Tilmicosin and preparation method thereof

A technology for tylosin and residue analysis, applied in analytical materials, measuring devices, instruments, etc., can solve the problems of complicated operation, long analysis time, high analysis cost, etc., and achieve a wide range, sensitive detection, and low false positive. Effect

Inactive Publication Date: 2012-07-18
CHONGQING NORMAL UNIVERSITY
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Problems solved by technology

Although the instrumental method has shown good performance in the identification of drug species and quantitative analysis, it is not suitable for residue screening of a large number of samples due to its complicated operation and high analysis cost
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  • Immune colloidal gold test strip for simultaneously carrying out residue detection and analysis on Tylosin and Tilmicosin and preparation method thereof
  • Immune colloidal gold test strip for simultaneously carrying out residue detection and analysis on Tylosin and Tilmicosin and preparation method thereof
  • Immune colloidal gold test strip for simultaneously carrying out residue detection and analysis on Tylosin and Tilmicosin and preparation method thereof

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[0024] Example

[0025] 1 Preparation of immunogen and coating

[0026] The immunogen and the DES-BSA synthesis method on the test strip detection line of the present invention. Weigh 21.9 mg of O-carboxymethyl hydroxylamine hemihydrochloride (AOAA) and 8.4 mg of sodium bicarbonate and dissolve them in 4 mL of tri-distilled water. The above solution was added dropwise to the DES solution (154 mg DES was dissolved in 4 mL of methanol), and the reaction was stirred for 3 hours at room temperature with magnetic stirring. After the reaction, it was rotary evaporated under reduced pressure until it contained a small amount of water, 4 mL of dichloromethane and 2 mL of absolute ethanol were added to redissolve, 5 g of anhydrous sodium sulfate was added, and the mixture was shaken and allowed to stand overnight. After filtration, it was rotated under reduced pressure at 60°C. Weigh 44.2 mg of the above-mentioned evaporated product, 12.4 mg of N,N-dicyclohexylcarbodiimide (DCC), and 6.9 mg

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Abstract

The invention discloses an immune colloidal gold test strip for simultaneously carrying out residue detection and analysis on Tylosin and Tilmicosin and a preparation method thereof. A cellulose nitrate film (4) is coated by a rabbit anti-rat IgG (Intravenous Gamma Globulin) quality control line (5) and a DES (Diethylstilbestrol)-BSA (Bovine Serum Albumin) detection line (6); and a binding cushion (8) is coated by monoclonal antibody-colloidal gold labels of an anti-TYL (Tylosin) and anti-TIL (Tumor Infiltrating Lymphocyte) drug. According to the preparation method provided by the invention, the monoclonal antibody of the anti-TYL and anti-TIL drug is screened by using an immune technology; and the colloidal gold test strip for simultaneously detecting TYL and TIL drug residues is built for applying the monoclonal antibody of the anti-TYL and anti-TIL drug and the colloidal gold technical research through preparation of colloidal gold and exploration research of a colloidal gold labeling condition, and then the properties of the colloidal gold test strip are tested. The test strip has the advantages of wider detecting sample range, low false positive, and sensitive, accurate and reliable detection, and is applicable to the multi-residue detection of the TIL and TYL drug residues in animal edible tissues, such as muscle tissues, liver tissues, kidney tissues and the like.

Description

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Claims

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Application Information

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Owner CHONGQING NORMAL UNIVERSITY
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