Application of polydatin in preparation of pharmaceuticals for preventing and treating chronic glomerular diseases

[0029] Example 1: Polydatin improves the treatment of glomerulopathy in fructose model rats.

[0030] Experimental animals: SD rats, 200-220 grams, male.

[0031] Drug preparation: use polydatin content of 20mg / tablet for the prevention or treatment of glomerular diseases, ultrasonically dispersed in normal saline, for intragastric administration, the dosage is 7.5mg / kg.

[0032] Experimental instruments: high-speed refrigerated centrifuge, tissue slicer, microplate reader, spectrophotometer, optical microscope, transmission electron microscope, etc.

[0033] Experimental model: Fructose rat model.

[0034] experimental method:

[0035] 1. Establishment of the model: After the animals adapt to the environment for 1 week, they are randomly divided into a model group and a normal group, with 30 animals in each group. Fructose rat model modeling: drinking water was given 10% fructose solution; the normal group was given tap water, the modeling cycle was 70 days.

[0036] 2. Four weeks late

[0048] Example 2: Polydatin improves the treatment of glomerulopathy in rats with glomerulosclerosis induced by adriamycin.

[0049] Experimental animals: SD rats, 200-220 grams, male.

[0050] Medicine preparation: use polydatin content of 20mg / tablet for the prevention or treatment of glomerular diseases, ultrasonically dispersed in normal saline, for intragastric administration, the dosage is 75mg / kg. Doxorubicin hydrochloride for injection (trade name: Famaxin Injection; Sufamaxia Company).

[0051] Experimental method: Focal segmental glomerulosclerosis is a common primary glomerular disease, mainly due to abnormal glomerular hemodynamics leading to podocyte damage, which is easy to progress to end-stage chronic renal failure .

[0052] 1. Establishment of the model: After the animals adapt to the environment for 1 week, they are randomly divided into a model group and a normal group, with 30 animals in each group. Rats in the model group were injected with adriamycin 2.5 mg /

[0062] Example 3:

[0063] According to the conventional preparation method, polydatin is added with water and an appropriate amount of solubilizer (polyethylene glycol 400) to dissolve, subpackage, and sterilize to prepare an oral solution for the prevention and treatment of chronic glomerular diseases with a 20mg / ml polydatin content ;

[0064] According to the conventional preparation method, polydatin and sorbitol are selected as soft capsule materials to prepare a capsule for the prevention and treatment of chronic glomerular disease with a content of 20mg / capsule of polydatin;

[0065] According to the conventional preparation method, the polydatin is added with the excipient cyclodextrin, mixed uniformly, granulated, and tableted to prepare a tablet for the prevention and treatment of chronic glomerular diseases with a content of 20 mg / tablet of polydatin.