The invention discloses a die cushion stroke regulating device for punch presses, which comprises a die cushion assembly, wherein the die cushion assembly comprises a die cushion supporting seat, a die cushion roof cover and an air cylinder, the air cylinder comprises a cylinder body, a piston and a piston rod, the air cylinder is arranged below the die cushion roof cover, and the top of the piston rod is fixedly connected with the lower end face of the die cushion roof cover; and the die cushion stroke regulating device also comprises a worm-and-gear mechanism and a speed reducing motor, wherein the worm-and-gear mechanism comprises a worm gear and a worm which are matched with each other, the worm is connected with an output shaft of the speed reducing motor, the piston rod is hollowly sleeved with a die cushion sawed tooth, the die cushion sawed tooth is in thread connection with the worm gear, the tail part of the piston rod is provided with a limiting nut, and the limiting nut is selectively pressed against and matched with the die cushion sawed tooth. According to the invention, mainly based on a principle of driving the die cushion sawed tooth to ascend and descend through the rotation of the worm gear and the worm, the stroke range of a die cushion is regulated so as to meet the different needs of users, therefore, the device can save cost, time and labor, and the device is high in operating efficiency, good in safety and applicable to industrialized promotion.