Sinonovacula constricta-derived bacillus alvei protein, application thereof to aquatic product preservation and application method thereof
A technology of bacillus and hive, which is applied in the field of razor clam derived bacillus hive protein, which can solve the problems of single species, slow development, and less application of biotechnology, so as to slow down the change of color and odor, slow down the rise, and have a good fresh-keeping effect Effect
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[0014] Detailed ways
[0015] A kind of razor clam source Bacillus alvei protein of the present invention is prepared according to the following steps:
[0016] a. Inoculate the production strain Bacillus alvei 110 (Bacillus alvei 110) in a liquid medium with glucose 40 g / L and peptone 20 g / L, pH 5, 150 mL or 200 mL per bottle Or 250 mL of liquid medium, temperature 30°C or 35°C, rotation speed 150 rpm, 200 rpm, or 250 rpm, shaker culture for 4 days, 5 days or 6 days. The Bacillus alvei was preserved on December 31, 2012 in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, with a preservation number: CGMCC No: 7079.
[0017] b. The antibacterial activity of the fermentation broth was detected by the filter paper method. Escherichia coli (EC), Proteus vulgaris (PV), Staphylococcus aureus (SA), Enterobacter aerogenes (EA) and Bacillus subtil
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