Sinonovacula constricta-derived bacillus alvei protein, application thereof to aquatic product preservation and application method thereof

A technology of bacillus and hive, which is applied in the field of razor clam derived bacillus hive protein, which can solve the problems of single species, slow development, and less application of biotechnology, so as to slow down the change of color and odor, slow down the rise, and have a good fresh-keeping effect Effect

Active Publication Date: 2014-08-06
DALIAN OCEAN UNIV +1
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the high price of enzyme preparations limits its popularization and application. Second, only a few natural microbial preservatives are all

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0014] Detailed ways

[0015] A kind of razor clam source Bacillus alvei protein of the present invention is prepared according to the following steps:

[0016] a. Inoculate the production strain Bacillus alvei 110 (Bacillus alvei 110) in a liquid medium with glucose 40 g / L and peptone 20 g / L, pH 5, 150 mL or 200 mL per bottle Or 250 mL of liquid medium, temperature 30°C or 35°C, rotation speed 150 rpm, 200 rpm, or 250 rpm, shaker culture for 4 days, 5 days or 6 days. The Bacillus alvei was preserved on December 31, 2012 in the General Microbiology Center (CGMCC) of the China Committee for the Collection of Microbial Cultures (CGMCC), located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, with a preservation number: CGMCC No: 7079.

[0017] b. The antibacterial activity of the fermentation broth was detected by the filter paper method. Escherichia coli (EC), Proteus vulgaris (PV), Staphylococcus aureus (SA), Enterobacter aerogenes (EA) and Bacillus subtil

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A sinonovacula constricta-derived bacillus alvei protein and application thereof aquatic product preservation and an application method. The preparation method is as below: culturing sinonovacula constricta symbiotic bacillus alvei strains in a liquid medium comprising 40g / L glucose and 20g / L peptone and having pH value adjusted to 5; filling 150-250 mL of the liquid medium into each bottle and conducting shaking culture at the temperature of 30-35 DEG C and speed of 150-250 rpm for 4-6 days; centrifuging at 4000 r / min to remove thalli in the fermentation liquid, carrying out decompression concentration at 50 DEG C until 1/2 of the original volume, adding ammonium sulfate until the final saturation concentration reaching 90%, standing overnight at 4 DEG C, filtering, dissolving the precipitate with a PBS buffer liquid, conducting dialysis by using a dialysis bag with the cut-off molecular weight of 3600 Dalton for 4 days, conducting freeze-drying on the products in the dialysis bag, so as to obtain the sinonovacula constricta-derived bacillus alvei protein. The preparation process provided by the invention is simple and low in cost.

Description

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Owner DALIAN OCEAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products