Method for preparing selaginella doederleinii hieron polysaccharide extract product and application thereof

[0017] Example 1 Preparation of Cypress polysaccharide extract

[0018] (1) Degreasing: take the dried whole herb of Cypress cypress, soak it with 8 times the amount of ethanol, reflux twice, filter, and evaporate the organic solvent to get the medicinal residue; (2) Extraction: soak the above medicinal residue with 10 times the amount of water, then soak in a water bath Extract 3 times, 1 hour each time, filter, concentrate the filtrate to 1.0g crude drug per ml; (3) Alcohol precipitation: add an appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place in ice bath for 12 hours, Filtration, precipitation and freeze-drying of the crude polysaccharide extract I; (4) protein removal: dissolve the above crude polysaccharide extract I in water to prepare a 0.05 g / ml crude product I solution, add 1 volume of chloroform-n-butanol ( V / V= 4:1) mixed solution, layered after stirring, layered after stirring, the upper layer was repeate

[0019] Example 2 Preparation of Cypress polysaccharide extract

[0020] (1) Degreasing: Take the dried whole herb of Cypress cypress, soak it with 10 times the amount of 80% ethanol, reflux twice, filter, evaporate the organic solvent to get the medicinal residue; (2) Extraction: soak the above medicinal residue with 8 times the amount of water , extracted twice in water bath, 1 hour each time, filtered, concentrated filtrate to 1.5g of crude drug per ml; (3) Alcohol precipitation: Add appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place in ice bath for 24 hours, filtered, precipitated and freeze-dried polysaccharide extract crude product I; (4) protein removal: dissolve the above polysaccharide extract crude product I in water to prepare a 0.1g / ml crude product I solution, add 3 times the volume of chloroform-n-butyl Alcohol (V / V= 4:1) mixed solution, stirred and layered, the upper layer was repeated 8 times, added

[0021] Example 3 Preparation of Cypress polysaccharide extract

[0022] (1) Degreasing: Weigh 4 kg of cypress herb (dry product, whole plant), soak in 8 times the amount of 80% ethanol for 3 hours (W / V), reflux at 85°C for 5 hours, twice. Filter, spread the dregs evenly, and evaporate the ethanol; (2) Extraction: evaporate the dregs above until there is no alcohol smell, add 4 times the amount of distilled water (W / V), soak for 12 hours, extract in a 50°C water bath for 2 hours, twice. Suction filtration through a four-layer gauze Buchner funnel, collect the filtrates, combine them, and concentrate to 3000ml by rotary evaporation at 60°C; (3) Alcohol precipitation: add about 9000ml of absolute ethanol to the above concentrated solution, and adjust to 80% ethanol content with an alcohol hydrometer , placed in ice bath for 24 hours, flocculent precipitate appeared, filtered with four layers of gauze Buchner funnel, precipitated freeze-dried crude polysaccharide extrac