Bacteria immobilization particles for water purification and preparation method of bacteria immobilization particles
A technology for water purification and bacteria, applied in chemical instruments and methods, immobilized on or in inorganic carriers, immobilized on/in organic carriers, etc. External environmental damage, poor mechanical properties of particles, etc., to achieve the effect of reducing bacterial loss, shortening start-up time, and enhancing stability
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[0036] Example 1
[0037] Bacterial liquid: Bacillus subtilis liquid.
[0038] The preparation method of Bacillus subtilis immobilized particles includes the following preparation steps:
[0039] (1) Add 1.6 kg of activated carbon and 0.4 kg of diatomaceous earth to 18 L of the bacterial liquid in the late logarithmic growth period, stir and absorb for 20 min, to prepare the Bacillus subtilis bacterial liquid;
[0040] (2) Add 160 mL of 12wt% chitosan acetic acid solution to the microbial broth prepared in step (1), stir quickly for 20 minutes, then slowly stir for 30 minutes, then let stand for 6 hours, and then suck up with a siphon The supernatant was made into a 3.8 kg bacterial concentrate;
[0041] (3) Add 2.4 kg of diatomaceous earth, 0.8 kg of sodium bentonite and 0.8 kg of silica to the bacterial concentrate prepared in step (2), and stir for 20 minutes;
[0042] (4) Add 6.24 L (15wt% PVA + 0.75wt% SA) mixed solution to the bacterial mixture obtained in step (3), stir the materia
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[0049] Example 2
[0050] The microorganism liquid is a mixture of Bacillus subtilis, Bacillus thuringiensis, yeast, and photosynthetic bacteria.
[0051] The preparation method of mixed bacteria immobilized particles includes the following preparation steps:
[0052] (1) Add 200 g of activated carbon and 50 g of diatomaceous earth to 2L of the bacterial mixture in the late logarithmic growth period (the mixing ratio of Bacillus subtilis, Bacillus thuringiensis, yeast, and photosynthetic bacteria is 2:1:1:1) In the medium, stirring and adsorbing for 20 minutes, the microorganism liquid is prepared;
[0053] (2) Add 20 mL of 12wt% chitosan acetic acid solution to the microbial broth prepared in step (1), stir quickly for 20 minutes, then slowly stir for 30 minutes, let it stand for 6 hours, and then suck it up with a siphon. Clear liquid to make 450 g bacterial concentrate;
[0054] (3) Add 300 g of diatomaceous earth, 100 g of sodium bentonite and 100 g of silicon dioxide to the bacter
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