A strain of Enterococcus faecium e9-10 and its screening and identification and its application in degrading spiramycin

A technology of Enterococcus faecium and spiramycin, which is applied in the detection and application of microorganisms, can solve the problems of human intestinal bacterial flora imbalance and change the micro-ecological environment of human intestinal flora, so as to reduce secondary pollution and cost low, efficiency-enhancing effect

Active Publication Date: 2018-09-18
山东中创健康科技集团有限公司
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Long-term use will change the micro-ecological environment of the human intestinal flora, causing the

Method used

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  • A strain of Enterococcus faecium e9-10 and its screening and identification and its application in degrading spiramycin
  • A strain of Enterococcus faecium e9-10 and its screening and identification and its application in degrading spiramycin
  • A strain of Enterococcus faecium e9-10 and its screening and identification and its application in degrading spiramycin

Examples

Experimental program
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Example Embodiment

[0063] Example, a strain of Enterococcus faecium E9-10, deposited in the General Microbiology Center of the Chinese Microbial Species Collection Management Committee, and its deposit number is: CGMCC No.10575.

[0064] The above screening method of Enterococcus faecium E9-10 includes the following steps:

[0065] 1 Experimental materials

[0066] 1.1 Experimental strains

[0067] Sample source

[0068] Collect soil samples from different locations in Weifang, Yantai and other regions

[0069] 1.2 Experimental equipment

[0070] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); biological safety cabinet (XUN BSC-1300ⅡA / B3); microscope (OLYMPUS CX21); high performance liquid chromatography (Aglient 1260); high performance liquid chromatography / mass spectrometry / Mass spectrometer (Aglient 1260-6430); analytical balance (Switzerland Mettler Toledo PL 602-S); constant temperature incubator (Germany MMM Incucell 111); pipette (Germany Eppendorf); desktop high-speed centrifuge (G

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Abstract

The invention discloses a strain of Enterococcus faecium, which is preserved in the General Microorganism Center of China Microbiological Strain Preservation Management Committee. Strains of lactic acid bacteria with spiramycin-degrading effect. Establish a reasonable and effective screening method for spiramycin-degrading strains to obtain suitable lactic acid strains. Through a series of work such as separation, purification and screening of lactic acid bacteria; primary screening and re-screening of spiramycin-degrading lactic acid bacteria, safe and effective spiramycin-degrading strains were screened. Applied to the treatment of spiramycin pollution, it has the advantages of no secondary pollution, high treatment efficiency, wide application range and low cost. Provide more options for microbial treatment of antibiotic pollution in complex environments.

Description

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Claims

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Application Information

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Owner 山东中创健康科技集团有限公司
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