Enzymolysis-assisted method for extracting cordycepin from waste Cordyceps militaris culture medium

[0018] Example 1

[0019] First, weigh 500g of the discarded Cordyceps militaris culture medium and put it into a tissue pulverizer to pulverize at a speed of 4000r / min, pass through a 50-mesh standard sieve, put the sieved culture medium fragments into a vacuum degasser for degassing treatment for 15 minutes, and then pass through a 50-mesh standard sieve. After the ultra-high pressure equipment is kept under the pressure of 250MPa for 1h, the waste medium scrap of Cordyceps militaris after pretreatment is obtained; then the above-mentioned waste medium scrap after pretreatment and distilled water are mixed according to the volume ratio of 1:3 and loaded Put it into a ceramic enzymolysis tank, add neutral protease with 1% of the total mass of the enzymolysis substrate, mix the substrate and protease evenly with a stirring rod, and adjust the pH to 5 with a citric acid solution with a concentration of 0.5mol / L. After sealing the mouth of the tank, put it in an incubator and i

[0021] Example 2

[0022] First, weigh 550g of waste Cordyceps militaris culture medium and put it into a tissue pulverizer to pulverize at a speed of 4500r / min, pass through a 55-mesh standard sieve, put the sieved culture medium fragments into a vacuum degasser for degassing treatment for 18 minutes, and then pass through a 55-mesh standard sieve. After the ultra-high pressure equipment is kept at a pressure of 300MPa for 1.5h, the waste medium scrap of Cordyceps militaris after pretreatment is obtained; then the waste medium scrap after the above pretreatment and distilled water are mixed at a volume ratio of 1:3 Put it into a ceramic enzymolysis tank, add neutral protease with 1.5% of the total mass of the enzymolysis substrate, mix the substrate and protease evenly with a stirring rod, and adjust the pH to 5.5 with a citric acid solution with a concentration of 0.5mol / L. After the membrane seals the mouth of the tank, put it in an incubator and incubate the enzymatic hyd

[0024] Example 3

[0025] First, weigh 600g of the discarded Cordyceps militaris culture medium and put it into a tissue pulverizer to pulverize it at a speed of 5000r / min and pass it through a 60-mesh standard sieve. Put the sieved culture medium fragments into a vacuum degasser for degassing treatment for 20 minutes, and then pass through a 60-mesh standard sieve. After the ultra-high pressure equipment is kept at a pressure of 350MPa for 2 hours, the pretreated waste medium scrap of Cordyceps militaris is obtained; then the above pretreated waste medium scrap and distilled water are mixed at a volume ratio of 1:3 and loaded Put it into a ceramic enzymolysis tank, add 2% neutral protease of the total mass of the enzymolysis substrate, mix the substrate and protease evenly with a stirring rod, and adjust the pH to 6 with a citric acid solution with a concentration of 0.5mol / L. After sealing the mouth of the tank, put it in an incubator and incubate the enzymatic hydrolysis a