Application of a squid ink preparation and cisplatin composition in the preparation of chemotherapy drugs for breast cancer

[0044] A preparation method of squid ink preparation with breast cancer chemotherapy synergistic activity, comprising the steps of:

[0045] (1) Take the frozen squid ink sac and thaw it at 4°C, take out the ink, add 1 times the volume of ice-cold phosphate buffer saline PBS, the pH of PBS is 7.2, and the concentration is 0.01M. 5s, 10s interval, 20 times;

[0046] (2) The ultrasonically treated suspension was stirred and soaked at 4°C for 48 hours, then centrifuged at 4°C for 60 minutes, and the speed of the centrifuge was 8000rpm;

[0047] (3) Remove the precipitate from the centrifuged liquid, add papain to the supernatant to a concentration of 0.15%, and incubate in a water bath at 55°C for 60 minutes;

[0048] (4) Heating in a water bath at 100°C for 20 minutes, after cooling, centrifuge at 4°C for 60 minutes, and the centrifuge speed is 8000rpm;

[0049] (5) Remove the precipitate from the above centrifuge, add 1 / 4 volume of chloroform and n-butanol mixture to the super

[0056] A preparation method of squid ink preparation with breast cancer chemotherapy synergistic activity, comprising the steps of:

[0057] (1) Take the frozen squid ink sac and thaw it at 4°C, take out the ink, add 3 times the volume of ice-cold phosphate buffer saline PBS, the pH value of PBS is 7.2, and the concentration is 0.01M. 5s, 10s interval, 20 times;

[0058] (2) The ultrasonically treated suspension was stirred and soaked at 4°C for 72 hours, then centrifuged at 4°C for 60 minutes, and the speed of the centrifuge was 8000rpm;

[0059] (3) Remove the precipitate from the centrifuged liquid, add papain to the supernatant to a concentration of 0.15%, and incubate in a water bath at 55°C for 60 minutes;

[0060] (4) Heating in a water bath at 100°C for 20 minutes, after cooling, centrifuge at 4°C for 60 minutes, and the centrifuge speed is 8000rpm;

[0061] (5) Remove the precipitate from the above centrifuge, add 1 / 4 volume of chloroform and n-butanol mixture to the

[0068] A preparation method of squid ink preparation with breast cancer chemotherapy synergistic activity, comprising the steps of:

[0069] (1) Take the frozen squid ink sac and thaw it at 4°C, take out the ink, add 2 times the volume of ice-cold phosphate buffer saline PBS, the pH of PBS is 7.2, and the concentration is 0.01M. 5s, 10s interval, 20 times;

[0070] (2) The ultrasonically treated suspension was stirred and soaked at 4°C for 60 hours, then centrifuged at 4°C for 60 minutes, and the speed of the centrifuge was 8000rpm;

[0071] (3) Remove the precipitate from the centrifuged liquid, add papain to the supernatant to a concentration of 0.15%, and incubate in a water bath at 55°C for 60 minutes;

[0072] (4) Heating in a water bath at 100°C for 20 minutes, after cooling, centrifuge at 4°C for 60 minutes, and the centrifuge speed is 8000rpm;

[0073] (5) Remove the precipitate from the above centrifuge, add 1 / 4 volume of chloroform and n-butanol mixture to the super