Application of senecio cineraria extract in preparing drug for treating deposition of liver fat

[0016] Embodiment 1: the preparation of snow chrysanthemum extract

[0017] 1. Soaking: Weigh a certain amount of snow chrysanthemum powder, soak it in 50-80% ethanol solution at a ratio of 1:8-20, and soak overnight.

[0018] 2. Ultrasonic and suction filtration: Ultrasonic the snow chrysanthemum ethanol solution soaked overnight, the temperature is 50-70°C, the time is 10-30min, and then the first suction filtration is performed. The snow chrysanthemum solid after the first suction filtration is soaked in 50-80% ethanol solution at a ratio of 1:8-20 and then ultrasonicated again at a temperature of 50-70°C for 10-30 minutes, and then the second suction filtration . The liquids after two suction filtrations were all poured into a flask for rotary evaporation to make an extract. Rotary evaporator (RE-3000 type, Zhengzhou Yarong Instrument Co., Ltd.), temperature 30-50°C, speed 100r / min (revolutions / minute). The final pH of the extract was 7.2-7.4.

[0019] Embodiment 2: The content of flavonoids in snow chrysanthemum is determined by high performance liquid chromatography

[0020] Precisely weigh 7 mg of the extract sample of Snow Chrysanthemum, put it in a 25 mL measuring bottle, add methanol to the mark, filter through a 0.45 μm microporous membrane, and perform high performance liquid chromatography detection. Chromatography using LC-20 AB Shimadzu high performance liquid chromatography (Shimadzu, Japan). Chromatographic column Inertsil ODS-SP column (4.6 mm × 150 mm, 5 μm); mobile phase is methanol-0.05% phosphoric acid aqueous solution; flow rate 0.6 mL min -1 , column temperature 25 ℃, detection wavelength 330nm, injection volume 10 μL, external standard method.

[0021] Embodiment 3: Determination of the content of chlorogenic acid in snow chrysanthemum by high performance liquid chromatography

[0022] Precisely weigh 7 mg of the extract sample of Snow Chrysanthemum, put it in a 25 mL measuring bottle, add methanol to the mark, filter through a 0.45 μm microporous membrane, and perform high performance liquid chromatography detection. Chromatography using LC-20 AB Shimadzu high performance liquid chromatography (Shimadzu, Japan). Chromatographic column Inertsil ODS-SP column (4.6 mm × 150 mm, 5 μm); mobile phase is methanol-0.05% phosphoric acid aqueous solution; flow rate 0. 6 mL min -1 , column temperature 25 ℃, detection wavelength 330nm, injection volume 10 μL, external standard method.