Improved adenovirus, construction method of system of improved adenovirus, and application of improved adenovirus

A construction method and adenovirus technology, applied in the field of adenovirus, can solve the problem of false positive virus affecting immunity and treatment effect, and achieve the effect of eliminating false positive effect

Active Publication Date: 2018-04-20
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The object of the present invention is to provide a construction method and application of an improved adenovirus and its system, which solves the problem that the virus particles in the adenovirus system in the pri

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  • Improved adenovirus, construction method of system of improved adenovirus, and application of improved adenovirus
  • Improved adenovirus, construction method of system of improved adenovirus, and application of improved adenovirus
  • Improved adenovirus, construction method of system of improved adenovirus, and application of improved adenovirus

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Embodiment

[0042] Example: Taking the preparation of human defensin hFIZZ3 monoclonal antibody as an example, the above-mentioned construction method of the present invention and related experimental descriptions are explained in detail

[0043] Design primers to amplify hFIZZ3 (about 300bp) from human cDNA, introduce NotI and XhoI at both ends of the gene, the primer sequence is: upstream primer Not I-hf3-F: 5'-TCTGCGGCCGCAG ATG AAG CGC GCA AGA CCG TCT-3' ; Downstream primer XhoI-hf3-R: 5'-GGC CTCGAG GGG CTG CAC ACG ACA GCA GCG CGC-3', digested and loaded into the transformed vector pShuttle-CMV-fiber to obtain pShuttle-CMV-fiber-hFIZZ3 After linearization, it was recombined with BJ-5183-AD bacteria containing the virus backbone, and the monoclonal recombinant was cultured to extract plasmids for enzyme digestion and identification, and the positive plasmid pShuttle-cmv-fiber-hFIZZ3-AD was obtained, and pShuttle- cmv-fiber-hFIZZ3-AD plasmid, recover a 30kbp DNA fragment, transfect the 30kb

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Abstract

The invention relates to an improved adenovirus, a construction method of the system of the improved adenovirus, and an application of the improved adenovirus. After a fiber gene is knocked out of anAD293 cell, not only an fiber protein is expressed on the surface of the adenovirus particles, a fusion probe of the fiber protein and a target protein is also expressed on the surface, and the falsepositive influences of only fiber protein virus particles are eliminated through only the fusion protein of the fiber protein and the target protein on the surface of the virus particles.

Description

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Claims

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Application Information

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Owner SHENZHEN UNIV
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