Recombinant hansenula polymorpha for expressing Zika virus structural protein E and construction method thereof

A technology of Hansenula polymorpha and Zika virus, applied in the direction of microorganism-based methods, viruses, viral peptides, etc., can solve the problems of non-expression and low efficiency of ZKE protein expression, and achieve low cost, good immunogenicity, Cultivate Simple Effects

Active Publication Date: 2018-06-01
NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION +1
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Problems solved by technology

The main difficulty may lie in: affected by the structure of the ZKE protein, the ex

Method used

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[0021] The present invention will be further described in detail below in conjunction with the drawings and embodiments.

[0022] (1) Construction of a pHMOXG-alpha-ZKE recombinant expression vector containing Zika virus structural protein E gene fragment 1. According to the reported Zika virus structural protein E gene information (GenBank: KU820899.2), artificially design the Zika to be expressed Virus structural protein E gene fragment (designed in May 2017), the two ends of which use amplification primers (primer 1 and primer 2, designed in July 2017) can add restriction site EcoRI (carbon end, see sequence underlined) Position) and restriction site NotI (nitrogen end, see sequence italic position). The sequence of the artificially designed Zika virus structural protein E gene fragment is shown in SEQ.ID.NO.1, which is artificially synthesized.

[0023] 2. After successfully transferring the plasmid (pMD18-T, Takara) containing the designed Zika virus structural protein E gene fr

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Abstract

The invention discloses recombinant hansenula polymorpha for expressing Zika virus structural protein E and a construction method thereof. According to the recombinant hansenula polymorpha, a gene segment of the artificially synthesized Zika virus structural protein E is introduced into hansenula polymorpha to obtain a recombinant bacterium for expressing the Zika virus structural protein E. A nucleotide sequence of the gene segment of the Zika virus structural protein E is shown as SEQ ID NO.1. The recombinant hansenula polymorpha can be used for producing the recombinant Zika virus structural protein E and has the advantages of simple purification steps, low cost and great industrial production potential.

Description

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Claims

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Application Information

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Owner NINGBO MUNICIPAL CENT FOR DISEASE CONTROL & PREVENTION
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