Method for purifying and concentrating composite biological active factors and concentrated liquid thereof
A technology of bioactive factors and growth factors, which is applied in the field of purification and concentration of compound bioactive factors and its concentrate, can solve the problems of inability to guarantee the effective activity of compound active factors, high cost of concentration and extraction, poor utilization rate, etc., and achieve delay Effect of skin aging, guaranteed nutrition and functional ingredients, low cost
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[0019] Example 1
[0020] The present invention provides a method for purifying and concentrating composite biologically active factors and a concentrated solution thereof, including the following steps:
[0021] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;
[0022] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform a pressure increase treatment, pressurize to 120 MPa, and hold for 5 minutes, then quickly release the pressure to normal pressure, and take out the biological tissue mixture;
[0023] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological tiss
Example Embodiment
[0032] Example 2
[0033] A method for purification and concentration of compound biologically active factors and a concentrated solution thereof include the following steps:
[0034] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;
[0035] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform pressure-increasing treatment, pressurize to 400MPa, and hold for 8 minutes, then quickly release the pressure to normal pressure, and take out the biological tissue mixture;
[0036] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological tissue mixture The clear liquid o
Example Embodiment
[0045] Example 3
[0046] A method for purification and concentration of compound biologically active factors and a concentrated solution thereof include the following steps:
[0047] S1, adding the biological tissue culture medium containing the compound biologically active factors to the organic solvent, and continuously stirring until uniform, to obtain the biological tissue mixture;
[0048] S2: Put the biological tissue mixture obtained in S1 into a pressure processor, perform pressure-increasing treatment, pressurize to 700 MPa, and hold for 10 minutes, quickly release the pressure to normal pressure, and take out the biological tissue mixture;
[0049] S3: Add the biological tissue mixture solution taken out in S2 to the centrifuge and perform centrifugation. After the centrifugation is completed, filter through a 100nm filter medium to remove residual biological tissue impurities in the biological tissue mixture solution to obtain a biological tissue mixture The clear liquid of t
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