Magnetic drug-loaded nano-liposome APT-MZF-NPs/DTX and preparation method thereof

A drug-loaded nano and liposome technology, applied in the medical field, can solve the problems of lack of treatment methods for patients, low selectivity of cancer cells, serious adverse reactions, etc., and achieve the goal of inhibiting cell growth, excellent magnetic responsiveness, and good killing effect Effect

Pending Publication Date: 2020-09-08
JIANGSU TAIZHOU PEOPLES HOSPITAL
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Problems solved by technology

However, after a median remission period of 18-24 months, most patients inevitably enter the stage of castration-resistant prostate cancer (CRPC), which lacks targeted treatments and has a high mortality rate
Chemotherapy represented by docetaxe

Method used

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Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] 1. Preparation and characterization of magnetic drug-loaded nanoliposome APT-MZF-NPs / DTX

[0018] 1. Preparation of magnetic drug-loaded nanoliposome APT-MZF-NPs / DTX

[0019] 1) Preparation of MZF@OA NPs

[0020] The high-temperature thermal decomposition method in the reference literature (Guo Ting. In vitro experimental research on the targeted diagnosis and treatment of ovarian cancer with shRNA / DDP magnetic nanoliposomes based on MFH technology [M]: [Master's Dissertation], Nantong: Nantong University, 2016) , to prepare MZF@OA NPs;

[0021] 2) Preparation of MZF-NPs loaded with DTX:

[0022] Weigh hydrogenated lecithin (100mg), cholesterol (25mg), DSPE-PEG2000 (18mg) into a 50mL round-bottomed flask, fully dissolve it with an appropriate amount of chloroform solution; weigh 15mg of DTX solid powder and dissolve it in the prepared MES buffer solution (0.02mol / L, PH=5.5), then added dropwise to a round-bottomed flask, ultrasonically oscillated and mixed, the

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Abstract

The invention relates to a magnetic drug-loaded nano-liposome APT-MZF-NPs/DTX and a preparation method thereof. An inner core of the liposome APT-MZF-NPs/DTX is magnetic manganese-zinc-iron nanoparticles (MZF@OA NPs) and a drug DTX, an outer layer of the liposome APT-MZF-NPs/DTX is an encapsulated lipid material, and the outer layer is modified with an aptamer A10-3.2. The preparation method comprises the following steps: firstly, preparing MZF@OA NPs, encapsulating the outer layers of the MZF@OA and the drug molecule DTX by using a lipid material, and then performing modifying with an aptamerA10-3.2 through an EDC/NHS chemical cross-linking method. According to the invention, the dosage form of traditional chemotherapeutic drug DTX is changed, and the liposome has the advantages of smallparticle size, high stability, good targeting property and the like. Under action of an external alternating magnetic field, the compound has a good killing effect on prostate cancer cells, can effectively inhibit growth of the prostate cancer cells and induce apoptosis of the prostate cancer cells, and has an effect superior to any single treatment.

Description

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Claims

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Application Information

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Owner JIANGSU TAIZHOU PEOPLES HOSPITAL
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