Magnetically-induced self-assembled electrochemical biosensor for sensitively detecting trace nickel ions and application of magnetically-induced self-assembled electrochemical biosensor

[0056] A method for sensitively detecting trace Ni of the present invention 2+ The magnetically induced self-assembled electrochemical biosensor, the construction process is as follows:

[0057] 1) The magnetic glassy carbon electrode (MGCE) is sequentially made of 0.3 μm and 0.05 μm Al 2 o 3 The powder is polished to the mirror surface, and then ultrasonically cleaned in ultrapure water, absolute ethanol, and ultrapure water in turn, and the clean MGCE is obtained after drying;

[0058] 2) The α-Fe prepared by the preparation example 2 o 3 / Fe 3 o 4 - Ultrasonic dispersion of Au nanocomposites in ultrapure water to prepare α-Fe at a concentration of 20mg / mL 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension, for subsequent use;

[0059] 3) Add 8μL of 20mg / mLα-Fe 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension was added dropwise to the clean MGCE surface, and after the liquid was dried at room temperature, MGCE / α-Fe 2 o 3 / Fe 3 o 4 -Au modified electrode, s

[0099] A method for sensitively detecting trace Ni of the present invention 2+ The magnetically induced self-assembled electrochemical biosensor, the construction method is as follows:

[0100] 1) The magnetic glassy carbon electrode (MGCE) is sequentially made of 0.3 μm and 0.05 μm Al 2 o 3 The powder is polished to the mirror surface, and then ultrasonically cleaned in ultrapure water, absolute ethanol, and ultrapure water for 1 min, and then dried to obtain a clean MGCE for use;

[0101] 2) The α-Fe prepared by the preparation example 2 o 3 / Fe 3 o 4 -Au nanocomposites were ultrasonically dispersed in ultrapure water and prepared as α-Fe at a concentration of 5 mg / mL 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension, for subsequent use;

[0102] 3) Dispense 8 μL with a concentration of 5mg / mLα-Fe 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension is added dropwise to the clean MGCE surface, and after the liquid is dried, MGCE / α-Fe 2 o 3 / Fe 3 o 4 -Au modif

[0110] A method for sensitively detecting trace Ni of the present invention 2+ The magnetically induced self-assembled electrochemical biosensor, its construction method:

[0111] 1) The magnetic glassy carbon electrode (MGCE) is sequentially made of 0.3 μm and 0.05 μm Al 2 o 3 The powder is polished to the mirror surface, and then ultrasonically cleaned in ultrapure water, absolute ethanol, and ultrapure water in turn, and the clean MGCE is obtained after drying;

[0112] 2) The α-Fe prepared by the preparation example 2 o 3 / Fe 3 o 4 - Ultrasonic dispersion of Au nanocomposites in ultrapure water to prepare α-Fe at a concentration of 25mg / mL 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension, for subsequent use;

[0113] 3) Dispense 8μL of 25mg / mLα-Fe 2 o 3 / Fe 3 o 4 -Au nanocomposite suspension was added dropwise to the clean MGCE surface, and after the liquid was dried, MGCE / α-Fe 2 o 3 / Fe 3 o 4 -Au modified electrode, spare.

[0114] 4) Add an equa