Application of bacillus in rice cadmium pollution remediation

[0036] The specific embodiment, the claims of the present invention are further described in detail, but it does not constitute any limitation to the present invention, and anyone who makes a limited number of modifications within the scope of the claims of the present invention is still protected by the claims of the present invention within range.

[0037] Unless otherwise specified, the following examples are conventional experimental methods and operating procedures in the art.

[0038] The gorilla Bacillus massiliogorillae described in this application is derived from the gorilla Bacillus massiliogorillae preserved in the Guangdong Provincial Microbial Culture Collection Center (GDMCC) on March 16, 2020, with the preservation number GDMCC No: 60979.

[0039] Embodiment 1, field test of YC9L promoting rice growth effect

[0040] Bacterial suspension preparation: ① Put the bacteria into LB liquid medium after activation, transfer to fresh LB liquid medium after overnight culture, and shake until OD600=1.0; ② Centrifuge the bacterial liquid in a centrifuge at a speed of Centrifuge at 8000rpm for 5min; ③Resuspend the bacteria in the same amount of sterile water, and apply by dipping the roots when transplanting rice seedlings. Test location: cadmium-contaminated farmland in Yinchangping, Renhua County, Shaoguan City, N: 25°05′44.04″N, E: 113°37′51.51″E. Test time: transplanting on August 16, 2021. The rice plant height and soil available Cd content were measured on August 26, the rice was harvested on November 25, and samples of roots, stems, leaves and grains were collected for analysis ( figure 1 and figure 2 ). Test treatment: divided into 2 treatments, treatment 1: application of farmers' conventional fertilizer; treatmen

[0045] Embodiment 2, the screening of YC9L bacterial strain, (1) separation take Guangzhou Tianhe green belt vegetation rhizosphere soil as screening soil sample, take by weighing 10g soil sample and add in the 250mL triangular flask (adding glass beads) that 90mL sterile water is housed, The shaker was shaken for 30 minutes. Fully disperse the bacteria and let it stand for 20-30s. Then in a water bath at 90°C for 10 minutes, the supernatant was taken for 10-fold incremental dilution, using 10-3-10-4 dilution, 100 μL of each gradient was drawn and spread on an LB solid plate, and incubated upside down at 37°C for 24 hours. Observe and according to the growth characteristics of the colony shape, size, color, etc., pick the colonies and purify them on the LB solid medium, and then save the isolated bacteria for future use. (2) Screening The ability of the isolated strains to produce IAA was determined by the Salkowski colorimetric method. The isolated bacteria were inoculated int