Composite microbial inoculum for preparing mushroom residues into matrix, and preparation method and application of composite microbial inoculum

[0044] Embodiment 1 preparation of composite microbial bacterial agent

[0045] (1) Thermophilic cellulose-degrading bacteria, thermophilic cellulose-degrading actinomycetes, and thermophilic cellulose-degrading fungi were respectively placed in beef extract peptone liquid medium, Gaoshi No. 1 liquid medium, and potato dextrose liquid medium, Incubate with shaking at 50°C and 200 rpm for 2 to 3 days.

[0046] (2) Thermophilic cellulose-degrading bacteria measure the OD value at 600nm, thermophilic cellulose-degrading actinomycetes and thermophilic cellulose-degrading actinomycetes measure the spore concentration in the bacterial liquid, and then use sterile water to dissolve the thermophilic fiber Dilute the OD value of the cellulose-degrading bacteria to 1.0 and then mix them into a bacteria-like liquid. Dilute the spore concentrations of the thermophilic cellulose-degrading actinomycetes and the thermophilic cellulose-degrading actinomycetes to 1×10, respectively. 7 Individ...

[0049] 1. High-temperature cultivation of strains

[0050] (1) Preparation of bacterial suspensions of different samples: take 10 g of fresh samples, put them into a conical flask containing 90 mL of sterile water, shake at 200 rpm for 30 min, filter, and dilute the filtrate gradient to make 10 -1 -10 -7 Concentration gradient bacterial suspension. (2) Coating: Bacteria selection 10 -5 、10 -6 、10 -7 Three concentration gradients, fungi choose 10 -2 、10 -3 、10 -4 Three concentration gradients, choose 10 actinomycetes -4 、10 -5 、10 -6 Three concentration gradients were applied to beef extract peptone solid medium, Martin's medium, and modified Gaoshi No. 1 solid medium respectively.

[0051] (3) High-temperature culture: Inverted and static culture in a biochemical incubator, the temperature is set at 50°C.

[0052] 2. Isolation and purification of thermophilic strains

[0053] The growing dominant strains on beef extract peptone solid medium, Martin's medium, and im...

[0075] According to the preparation method of compound bacterial agent in embodiment 1, Bacillus urea (CGMCC1.15355), Acetobacter pasteuriani (CGMCC1.2269), Brevibacillus thermoruborus (CGMCC1.8649), Bacillus suffolus (CGMCC1.9052 ), Aspergillus fumigatus (CGMCC3.15720), and Thermomyces sp. (CGMCC3.17993) were cultured in the corresponding medium respectively, and all bacterial solutions were mixed in equal volumes 1:1:1 (V:V:V) into a composite microbial agent. All the above strains were purchased from China General Microorganism Culture Collection and Management Center.