Citrus DNA rapid extraction method

[0033] Example:

[0034] A rapid extraction method for citrus DNA, comprising the following steps:

[0035] 1) Rinse the citrus leaves with running water, dry the water with paper, and cut 0.1-0.2cm 2 Plant tissue, put it into a 2mL sterile centrifuge tube, and put 2 6mm sterile steel balls into it, add 1200uL of extraction solution (DNA extraction buffer containing 2.5% polyvinylpyrrolidone: lysis solution: 5% lauryl sarcosine The volume ratio of sodium = 3:3:2; the DNA extraction buffer, the components are: 20mM EDTA (pH 8.0), 350mM sorbitol, 100mM Tris-HCl (pH 8.0); the lysis solution, the components It is: 50mM EDTA (pH 8.0), 2M sodium chloride, 2% CTAB, 200mM Tris-HCl (pH 8.0)), cover the tube cap, put it into the shaking sampler, shake for 15min to obtain a homogenized tissue;

[0036] 2) Take 800uL of homogenized tissue, transfer it into another new 2mL sterile centrifuge tube, add 800uL of phenol / chloroform / isoamyl alcohol mixture (Tris saturated phenol:chloroform:i