Stable creatine beverages
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Example 1: Preparation of Ready-to-Drink Beverages
[0139]The following two ready-to-drink beverage formulations were prepared by mixing all ingredients until they dissolved completely. The beverage was then mixed for a further 15 minutes. The beverage was then heat treated at 195+ / −2° F. for 21-23 seconds, filled at 180° F. to 185° F. and cooled rapidly to room temperature.
[0140]Sports Beverage Formulation 1
IngredientAmount in Beverage (ppm-mg / L)Creatine Monohydrate2100Contributes ~1600 mg / L creatineBranched Chain Amino Acids2224Citric Acid1599Sodium Chloride1065Contributes 630 mg / L SodiumTri-sodium Citrate785Natural Flavor1582Potassium Phosphate541Contributes 160 mg / L PotassiumSucralose323Ascorbic Acid 50Magnesium Chloride42.4Contributes 5 mg / L MagnesiumCalcium Chloride38.7Contributes 10 mg / L CalciumAsulfame Potassium32.3Niacin15.1Vitamin B61.88Vitamin B129.90 micro gramsCalcium Disodium24 EDTA
[0141]Sports Beverage Formulation 2
IngredientAmount in Beverage (ppm-mg / L)Cre
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Example 2: Stability Study of Ready-to-Drink Beverages
[0142]The stability of the two ready-to-drink beverages described Example 1 was studied. The beverages were allowed to stand at 5° C. or 21° C. for the period of time indicated. Creatine was measured by HPLC with UV detection according to the method provided in Analytical Biochemistry 2014, pp. 278-283 (1993). Briefly, reversed-phase chromatography on a C18 column by employing gradient elution and UV detection at 210 nm was utilized. Separation was achieved in less than 5 minutes. The results are provided in Tables 1 and 2, below:
TABLE 1Creatine Monohydrate (mg / L)Sports BeverageSports BeverageFormulation #1Formulation #2Time (weeks)5° C.21° C.5° C.21° C. 016061772 4162595217391022 91598942172810181314909221666 9972612768661354 942
TABLE 2Creatine Monohydrate (% retention)Sports BeverageSports BeverageFormulation #1Formulation #1Time (weeks)5° C.21° C.5° C.21° C. 0100%100% 4101%59%98%58% 9100%59%98%57%13 93%57%94%56%26 79%54%76%53%
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