6 results about "Cell growth" patented technology

The invention discloses a culture medium for hepatoma organ cell sphere culture. The culture medium is composed of a basic culture medium, 10-25 ng/mL of an epidermal growth factor, 10-25 ng/mL of a fibroblast growth factor, 0.5-2 ng/mL of a cell growth factor, 1-3 ng/mL of an insulin growth factor, 1 mmol/L of sodium pyruvate, 2 mmol/L of glutamine, 100 U/mL of penicillin, 100 [mu]g/mL of streptomycin and 10% by volume of serum. Aiming at the culture growth characteristics of liver cancer tissue-derived cells, the culture medium provided by the invention is simple and reasonable in components, rich in nutrition and moderate in formula price; and hepatoma organ cell spheres cultured by the culture medium can effectively and stably grow for a long time, are closely gathered, have smooth andround surfaces and clear boundaries, is free of disintegration phenomenon, and can be significantly improved in cell activity, specific functions and metabolic functions.

The invention relates to an oral cavity defect repair membrane and a preparation method thereof, in particular to an acellular matrix material and preparation thereof, and the application in the repair of oral cavity defect. The preparation comprises: (1) taking the porcine small intestine jejunum segment, cleaning, radial cutting, removing the mucosa layer, sarcolemma layer and serosa layer, andobtaining the porcine small intestine submucosa tissue membrane; (2) soaking the tissue membrane of the porcine small intestine submucosa obtained in the step (1) in peroxyacetic acid solution or sodium hydroxide solution, and take out and cleaning to obtain an acellular matrix of the porcine small intestine submucosa tissue; (3) soaking the porcine small intestinal submucosa tissue obtained in the step (2) with a dopamine solution, sterilize, and obtaining an acellular matrix material. The invention can avoid the loss of cell growth factor in the tissue membrane of the porcine small intestinesubmucosa, ensure the the obtained acellular matrix material has better tissue micropore structure and mechanical properties, and can meet the needs of oral bone injury repair; low immunogenicity andgood safety.

The invention discloses a wild ganoderma product for resisting cancer and tumor and removing liver toxin and a preparation technology. According to the invention, wild ganoderma lucidum is adopted asraw material, and the wild ganoderma product for resisting cancer and tumor and removing liver toxin is finally prepared by the steps of washing, sterilizing, drying and dicing and the like. The product has the advantages of inhibiting cancer cell growth, attending acute and chronic hepatitis, early cirrhosis, esophageal cancer, lung cancer, liver cancer, nasopharyngeal carcinoma, pancreatic cancer, neurasthenia, intestinal diarrhea, rheumatoid tuberculosis,diabetes, gastrointestinal diseases, nourishing physical, lowering blood sugar, moistening the lungs and kidneys, maintaining beauty and keeping young, enhancing immunity, and enriches in ergosterol, ganoderic acid, ganoderma lucidum polysaccharides, amino acids, polypeptides, adenosine, triterpenoids and various trace elements, whereinthe organic germanium content is as high as 12380 ppm and the organic selenium content is also high. The product can be made into five-color ganoderma lucidum tea, ganoderma lucidum beverage, ganoderma lucidum oral liquid, five-color ganoderma lucidum wine, and other homologous products of medicines and foods. The product provided by the invention can also be made into traditional Chinese medicine prescriptions such as paste, pill and pellet.

The invention provides antibacterial dressings for promoting healing of skin wounds. The antibacterial dressings for promoting healing of the skin wounds are sponge-like body tissues formed by mixingaliphatic polyester, collagen, antibacterial medicines and cell growth-promoting factors and then performing freeze drying, wherein the aliphatic polyester, the collagen, the antibacterial medicines and the cell growth-promoting factors are uniformly distributed in the sponge-like body tissues. The dressings disclosed by the invention have the advantages of high compatibility, strong antibacterialproperty, multiple functions and quick response, and has strong bacteriostasis on gram-negative bacteria, gram-positive bacteria and fungi; moreover, the permeability of cell walls can be changed, and leakage of substances in the cells is caused, so that the bacteria are broken.

The serum-free culture of normal human epithelial stem cells is of paramount importance for the in vitro formation of cloned human tissues by means of cell therapy. Several growth promoting agents are disclosed for use in a serum-free culture medium of normal human keratinocytes. Lithium ions, dibutryl-cyclic adenosine monophosphate, and prostaglandin E1 have been found effective as growth enhancing agents to be added singly or in combination, when used in combination with insulin-like growth factor-1. Lithium ions and prostaglandin E1 are disclosed as independent growth enhancing factors, that replace epidermal growth factor as a necessary growth factor required for keratinocyte clonal growth.

A method for detecting cancer in a patient. The method comprises the steps of introducing labeled antibodies or labeled AFP to a biological sample of the patient so the labeled antibodies or labeled AFP will react with the AFP receptor binding sites in the biological sample. Next there is the step of identifying AFP receptor binding sites in the biological material which are reacted with the labeled antibodies or labeled AFP to determine the presence of cancer. Preferably, before the introducing step, there is the step of obtaining a biological sample from a body of a patient. A method for treating cancer cells in a patient. The method comprises the steps of introducing AFP receptor antibodies to cancer cells in the patient. Then there is the step of reacting the AFP receptor antibodies with the AFP receptor of the cancer cells to inhibit growth of the cancer cells or kill the cancer cells. A method for monitoring a patient. The method comprises the steps of treating the patient for cancer. Then there is the step of testing the patient at predetermined intervals after the treatment for AFP receptor site levels. A method for treating a patient. The method comprises the steps of testing the patient for AFP receptor. Then there is the step of introducing AFP receptor antibodies or AFP into the patient to react with cancer cells in the patient if the testing indicates AFP receptor are in the patient. A method for treating cancer cells in a patient. The method comprises the steps of introducing modified AFP to cancer cells in the patient. Then there is the step of reacting the modified AFP with the AFP receptor of the cancer cells to inhibit growth of the cancer cells or kill the cancer cells.