5 results about "Cotyledon" patented technology

The invention discloses a culture method for a brassica oleracea L. var. acephala microspore regeneration plant. The method comprises the following steps: taking inflorescences of brassica oleracea L. var. acephala and rape, directly taking or taking after induction alabastrum of the inflorescences, adding the alabastrum into NLN-13 induced medium after disinfection to form fluid suspension, carrying out filtering, and centrifuging the filtrate to obtain a precipitate; adding NLN-13 induced medium and active carbon mixed liquor in order so as to obtain a microspore fluid suspension; carrying out a heat shock treatment, followed by culturing so as to obtain embryoids in cotyledon stage; inoculating the embryoids to embryoid differential medium until the embryoids are differentiated and regenerates into buds; cutting the regenerated buds to a rooting medium for rooting culture, and hardening and transplanting seedlings to obtain regenerated plants; taking young leaves of the regenerated plants for the detection of ploidy of corresponding regenerated plants and determining regenerated seedlings of rape and brassica oleracea L. var. acephala in the regenerated plants. According to the method provided in the invention, rape which is easy to generate embryos and brassica oleracea L. var. acephala which is difficult to generate embryos are mixedly cultured; the material which is easy to generate embryos is used to spur the material which is difficult to generate embryos; therefore the ratio of embryos of brassica oleracea L. var. acephala is improved.

The invention discloses a method for cultivating peanuts with excellent characters, and belongs to the technical field of peanut breeding. According to the method, a cotyledon cutting method is adopted to cut off part of cotyledons of peanut early-stage hybridization segregation generation individuals, the cotyledons are used for measuring excellent characters, early-stage hybridization segregation generation groups are screened according to measurement results, and finally peanuts with excellent characters and stable inheritance are cultivated. In addition, a cotyledon cutting method is used for screening the excellent characters of the single-grain segregation generation group in the early hybridization stage, the problem that passage cannot be carried out after character detection of the single-grain peanut is solved, the breeding scale is reduced, the labor and time cost of breeding is greatly reduced, and the selection efficiency is improved. Meanwhile, the germination rate of the peanuts without cotyledons is not affected.

The invention discloses a continuous culture method for watermelons. Pumpkin seedlings or gourd seedlings serve as rootstock seedlings, watermelon seedlings serve as scions, and the cut-in method is adopted for grafting. The method concretely comprises the following steps that (1) seedling culture is performed on pumpkin seeds or gourd seeds through nutrition pots 6-8 days in advance, and then watermelon seeds are sowed, wherein the time when cotyledons of the watermelon seedlings are expanded and first true leaves of the rootstock seedlings emerge is the time suitable for grafting; (2) on a sunny morning or afternoon with strong sunlight, the rootstock seedlings proper in size are selected, growing points are removed from the rootstock seedlings through a blade, and a hole is formed by inserting the top of each rootstock seedling through a bamboo stick, wherein the depths of the holes is 1.0 cm; afterwards, the watermelon seedlings are taken and obliquely cut through the blade at the position of 1.5 cm from the lower portions of the cotyledons, wherein the length of the cuts is roughly equal to the depth of the holes; next, the obliquely cut water seedlings are inserted in the holes of the rootstock seedlings, so that grafting is completed. According to the continuous culture method for the watermelons, the problems that water melon seedlings based on a traditional water melon continuous culture method are susceptible to wilt disease, so that the water melon seedlings are dead in a large area, and meanwhile diseases and insect pests frequently occur are solved; besides, the method has the advantages of being capable of effectively preventing the wilt disease of the watermelon seedlings, enhancing stress resistance, promoting growth and increasing watermelon output.

The invention discloses a radish generation-adding seeding method. The method mainly comprises the step that a simple transplanting method of germinated radish seeds is adopted, wherein a 1.2-meter flat bed is selected, water is poured into the bed surface thoroughly, after the water percolates down, blunt-ended forceps are adopted for clamping root tails of buds and pressing them into mud, and cotyledons and the portion 2-3 mm away below the cotyledons are reserved and exposed above the ground. Operation is easy, and the survival rate is high. Due to the fact that links such as ditching or digging, bottom water irrigation and earthing are reduced, the efficiency is doubled compared with that of a conventional method.

The invention discloses a double-rolling brush type seedling trimming mechanism configured on a solanaceae automatic grafting machine and belongs to gardening vegetable seedling culture, production and grafting equipment. A height position adjustment motion assembly is fixedly arranged on a longitudinal insertion motion assembly, a transverse spacing adjustment motion assembly is assembled on the height position adjustment motion assembly, two transverse opposite side parts of the transverse spacing adjustment motion assembly are respectively provided with a motor, two axle type rolling brush rolls are parallel to each other and are respectively assembled on the motors at two sides in horizontal cantilever structures, supporting plates provided with strip-shaped slideway supporting holes are configured on the outer sides of the cantilever tail ends of the axle type rolling brush rolls, and the tail ends of the two axle type rolling brush rolls and the strip-shaped slideway supporting holes on the supporting plates are in inserting and supporting fit or separate fit. The double-rolling brush type seedling trimming mechanism can effectively remove vegetable seedling cotyledon by rows at high speed, and has the characteristics of simple structure, reliable running, simpleness and convenience in operation, and high success rate of grafting.