Method for cultivating peanuts with good characters
A technology of excellent traits and breeding methods, applied in the field of cultivation of peanut excellent traits, can solve the problems of shortening the breeding period, low detection efficiency, and speeding up the breeding process, so as to reduce the breeding scale, improve the selection efficiency, and reduce labor and time costs. Effect
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Embodiment 1
[0047] Two small peanut varieties, Huayu 20 and Huayu 23, were selected, and first-class rice with slightly whitish seed coats and full grains was selected, and the cotyledons were cut off according to the above-mentioned "cotyledon cutting method". Among them, when the excised length of the two cotyledons was 1 / 4, the average weights of the excised cotyledons of Huayu 20 and Huayu 23 were 99.76 mg and 118.41 mg, respectively, which could meet the drying requirements for the detection of oil, fatty acid or total sugar content. material weight. The germination test was performed on the seeds after excision of part of the cotyledons and the whole seeds. The seeds were placed in a petri dish with a diameter of 15 cm for indoor germination experiments. Spread filter paper at the bottom of the petri dish, put 20 seeds in each dish, soak the seeds with sterilized ultrapure water for 8 hours at room temperature, and then place them in a 25°C incubator for cultivation. After 7 days, t
Embodiment 2
[0052] Two large peanut varieties, Huayu 33 and Huayu 917, were selected, and first-grade rice with slightly whitish seed coats and full grains was selected, and the cotyledons were cut off according to the above-mentioned "cotyledon cutting method". Among them, when the excised length of two cotyledons was 1 / 4, the average weights of the excised cotyledons of Huayu 33 and Huayu 917 were 227.11 mg and 201.80 mg, respectively, which could meet the requirements for the detection of oil, fatty acid or total sugar content. material weight. The germination test was performed on the seeds after excision of part of the cotyledons and the whole seeds. The seeds were placed in a petri dish with a diameter of 15 cm for indoor germination experiments. Spread filter paper at the bottom of the petri dish, put 20 seeds in each dish, soak the seeds with sterilized ultrapure water for 8 hours at room temperature, and then place them in a 25°C incubator for cultivation. After 7 days, the germi
Embodiment 3
[0058] In the spring of 2010, the high-yielding line P10-2 (027×SIP009) was used as the female parent, and the high-oil peanut variety Xuhua No. 8 (8034-5×Luhua No. 6) was used as the male parent. 1 . Winter 2010, will F 1 Generation planted in Sanya, and harvested F 2 . In the spring of 2011, cut off two cotyledons at the end away from the germ, at 1 / 4 of the length of the seed. According to the above-mentioned method for measuring the oil content of cotyledons, the oil content of the cut parts of the two cotyledons was measured. Choose F with oil content ≥ 50% 2 Seeds were germinated indoors 7 days before planting. When the seeds were white and the roots elongated about 1.5cm, they were transplanted to the Laixi field, and the survival rate reached 100%. Harvest F 3 generation. In the spring of 2012, detection of single F 3 The oil content in the seeds, select the seeds with oil content above 50%, germinate the seeds indoors 7 days before planting, and transplant the
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