The invention discloses sesame oil with an efficacy of protecting eyes and a preparation method of the sesame oil. The sesame oil is prepared from the following raw materials in parts by weight: 1000-1100 parts of white sesames, 30-40 parts of red beans, 20-30 parts of ocimum basilicum seeds, 4-5 parts of evening primrose, 20-25 parts of carrot skin, 5-7 parts of wolfberry fruits, 10-15 parts of spinach, 3-4 parts of pork liver, 40-50 parts of sweet purple potatoes, 5-10 parts of oyster mushrooms, 2-3 parts of duck yolk, 4-5 parts of raw walnut kernels, edible alcohol and proper amount of water. According to the sesame oil with the efficacy of protecting eyes, white sesame is adopted as a main raw material, and the added wolfberry fruit, spinach, pork liver, sweet purple potatoes, oyster mushrooms, duck yolk, raw walnut kernels and the like have a certain eye protecting effect; the sesame oil has a certain eye protecting effect after being eaten for a long time, is heavy in flavor, retains the health nutritious components in raw materials, and has a very good health protection effect.