The invention relates to a preparation method of transglucosidase. The technical problem that during
isomaltose hypgather production, the
glucoside conversion rate is low is mainly solved. In order to solve the problem, according to the technical scheme, the
aspergillus niger strain (General
Microbiology Center of China Committee for Culture Collection of Microorganisms, CGMCC No.3.6478) serves as the original strain, a liquid
fermentation culture medium is adopted, inductors are specially added, the
aspergillus niger strain is induced to secrete a large amount of efficient transglucosidase through the four steps of slope cultivation,
shake flask expanded cultivation, liquid deep
fermentation and
centrifugal separation, and then high-density transglucosidase
fermentation liquid is prepared. The product can be applied to the key step, namely, the conversion stage, in
isomaltose hypgather production.