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2 results about "Light sensitivity" patented technology

Light sensitivity (photophobia or photosensivity) is a condition where your eyes are overly sensitive to light, possibly causing pain, tearing and discomfort. When light enters the eyes, it causes a chemical reaction in the back of the eyes. This reaction, effecting the rods and cones, allows us to adapt to differences between light and dark.

Chemiluminescence sensing method based on dsDNA-SYBR Green I light-sensitive catalysis

InactiveCN110241180AHigh sensitivityLow costMicrobiological testing/measurementSinglet oxygenBrca1 gene
The invention relates to a chemiluminescence sensing method based on dsDNA-SYBR Green I light-sensitive catalysis. Mainly, the oxidation capacity of singlet oxygen which is short in life and high in oxidation capacity and produced through light sensitivity of dsDNA-SYBR Green I is stored through K4Fe(CN)6, and the singlet oxygen is oxidized into K3[Fe(CN)6]. Through the simple storage step, the chemiluminescence intensity of light-sensitive oxidation luminal can be about 30 times, so that the sensitivity for detecting DNA can be notably improved, a detection limit of a BRCA1 gene can reach 3 pM, a detection limit of a BRCA2 gene can reach 6pM, and a detection limit of a p53 gene can reach 5pM. The method is simple and convenient to operate, does not need steps of separation, marking and the like, can be expanded to detection of DNA of any other sequence, and has favorable application prospects.
Owner:CHENGDU UNIVERSITY OF TECHNOLOGY
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