The invention relates to a
chemiluminescence sensing method based on dsDNA-
SYBR Green I light-sensitive
catalysis. Mainly, the oxidation capacity of
singlet oxygen which is short in life and high in oxidation capacity and produced through
light sensitivity of dsDNA-
SYBR Green I is stored through K4Fe(CN)6, and the
singlet oxygen is oxidized into K3[Fe(CN)6]. Through the simple storage step, the
chemiluminescence intensity of light-sensitive oxidation luminal can be about 30 times, so that the sensitivity for detecting
DNA can be notably improved, a
detection limit of a BRCA1
gene can reach 3 pM, a
detection limit of a BRCA2
gene can reach 6pM, and a
detection limit of a p53
gene can reach 5pM. The method is simple and convenient to operate, does not need steps of separation, marking and the like, can be expanded to detection of
DNA of any other sequence, and has favorable application prospects.