5 results about "Tumor Sample" patented technology

The invention discloses a method for removing a human gene sequence in macro genome sequencing data. The method comprises the following steps that a reference gene set is constructed through originalsequencing data of genome planning samples of thousands of people, and after high-quality sequencing read of genome data of thousands of people is obtained, genome assembly software assembles the sequencing read into a long gene segment to serve as a reference sequence later to be compared with the sequencing read; gene fragment data is extracted from all non-tumor samples from an NCBI database toserve as gene fragment data of an NCBI Bioproject for subsequent processing; the data of the genome data of thousands of people and the data of the NCBI Bioproject are merged, and after redundancy elimination, the data becomes a non-redundant gene fragment data set; virus genome sequences in the non-redundant gene fragment data set are found out and removed from the gene fragment sequence; the gene fragment sequence without the virus genome sequences serves as a reference genome for removing a human sequence in the macro genome sequencing data.

The present invention includes methods for treating a FLT3 mutated proliferative disorder comprising: measuring expression of a mutated FLT3 and one or more genetic abnormalities in a sample obtained from a tumor sample obtained from the patient, wherein the presence of the one or more genetic abnormalities indicates that the patient has a poor prognosis; and administering to the patient a therapeutically effective amount of crenolanib or a pharmaceutically acceptable salt thereof, wherein the crenolanib increases a chance of survival of the patient having both the mutated FLT3 and the one or more genetic abnormalities, wherein the crenolanib, as shown below, is administered to a subject suffering from said disorder:

The invention proposes a tumor sample pretreatment method. The tumor sample pretreatment method comprises the steps that a fresh tumor sample is subjected to first cleaning treatment in a cleaning buffer solution; the sample subjected to first cleaning treatment is removed so as to remove fat, blood, necrosis and tissue with mesenchyme mass fraction being greater than 18-22%; and the removed tissue is subjected to second cleaning treatment. According to the tumor sample pretreatment method, the microbial contamination probability after tumor sample culturing is effectively reduced, the culturesuccess rate of the tumor sample is increased, in the method, the fat, the blood, the necrosis and the tissue with the mesenchyme mass fraction being greater than 18-22% are selectively removed, theremoval standard is strictly limited, on the one hand, partial pollution can be removed, on the other hand, second cleaning is more thorough and more effective, obtaining of the tissue part with morecontent of tumor cells is also greatly improved, the subsequent enzymolysis efficiency is improved, and the subsequent number of clones successfully cultured through the tumor cells is increased.

Methods and compositions are provided for determining TMB in a tumor sample using transcriptome profiling data. Also provided herein are methods and compositions for determining the response of an individual with a specific TMB to a therapy such as immunotherapy.