4 results about "Vascular endothelium" patented technology

The invention discloses an improved culture medium for neonatal pig islet cells and a use method of the same. An apoptosis inhibitor Z-VAD-FMK, a human basic fibroblast growth factor hFGF-beta, a vascular endothelial growth factor VEGF, an insulin-like growth factor R3-IGF-1, a human epidermal growth factor hEGF, a hepatocyte growth factor hHGF, pig serum, sodium selenite, insulin, transferrin, ethanol amine, 1-methyl 3-isobutyl xanthine, nicotine, cortisol, penicillin and streptomycin are added in HAM'S F10 culture medium. According to the culture medium, apoptosis can be reduced and recovery rate can be increased; the cultured cells have a sensitive glucose stimulation response and more mature functions compared with generally-cultured islet cells; the insulin secretion amount of the islet cells with the same quantity is increased; the viability of islet beta-cells is improved; the breakages of islet cell clusters are reduced, and the envelops of the cell clusters are complete, so that the cell clusters are more suitable for being transplanted.

The invention relates to an application of an up-regulating agent of a homologous recombination repair (HR) or non-homologous end joining (NHEJ) pathway in preparation of a medicament for treating diabetes and preventing diabetic individual tumorigenesis. Inventors find that DSB and DSB repair mechanism damage exist in diabetic human cells and tissue, and confirm that EWS/Fbxw7 repairs damaged DNAby activating the HR pathway and the NHEJ pathway for the first time, and the cell cycle is blocked at G0/G1 phase to facilitate DNA repair; the EWS/Fbxw7 can significantly reduce the excessive activation of PARP in diabetic large blood vessels, microvascular endothelium and retinal nerve tissue; the mechanism that inhibits the excessive activation of the PARP can be related to the activation ofthe HR and NHEJ pathways; and results suggest that the up-regulating agent of the HR or NHEJ pathway can be used to prepare the medicament for alleviating a diabetes mellitus vascular endothelial dysfunction, or strengthening the DNA damage repair, or treating the diabetes mellitus, or preventing diabetic complications, or preventing the diabetic individual tumorigenesis.