2 results about "Wound infection" patented technology

The invention discloses a rapid transgenosis method taking citrus seedlings planted in soil as materials. The rapid transgenosis method comprises the following steps: preparation of explants, preparation of agrobacterium infection liquid, wound infection of seedlings and molecular biological identification of buds to be transformed, wherein the preparation of the explants is that the seedlings planted in soil are directly taken as the explants to transform so as to obtain transgenic plants. According to the rapid transgenosis method disclosed by the invention, test-tube stock seedlings are not needed to be prepared, buds to be transformed are not needed to be adapted, and the seedlings planted in soil are directly wounded and transformed to obtain buds; and moreover, planting of seeds in soil in a greenhouse is not affected by the culminate and the weather, and can be performed all the year round. The rapid transgenosis method has the advantages of simple and convenient operation steps, low cost and short time.