Preparation method for vacuum drying of transglutaminase

[0027] The preparation method of vacuum-drying transglutaminase of the present invention comprises the following steps: adding a thermoprotectant to the fermented liquid of transglutaminase, standing at room temperature, obtaining supernatant through high-speed refrigerated centrifugation, and using 95% ethanol to The supernatant is precipitated, and the precipitate is obtained by high-speed refrigerated centrifugation, and an antioxidant protection agent is added, mixed evenly, and vacuum-dried to obtain a transglutaminase enzyme preparation; wherein, the heat protection agent is a sugar alcohol substance, including sorbic acid Sugar alcohols and maltitol, antioxidant protection including active peptides.

[0028] Wherein, the enzyme activity of the transglutaminase in the transglutaminase fermented liquid is 10-50 u / ml.

[0029] In the present invention, the added amount of sorbitol is 0.5%~5%w / v, preferably 0.5%~2%w / v. The added amount of maltitol is 1%~10%w / v, prefer

[0036] to Streptomyces mobara ( Streptoverticillium mobaraense) into 100ml of transglutaminase fermentation broth produced by adding ethanol at a volume ratio of 0.5:1 ethanol:fermentation broth to precipitate the enzyme, and set the high-speed refrigerated centrifuge at 6000r / min, 4°C, and centrifuge for 20min to obtain the precipitate put the precipitate into a vacuum drying oven, and dry at 40° C. for 3 hours to obtain a transglutaminase preparation. Seal it with a ziplock bag, and measure the enzyme activity regularly.

[0038] to Streptomyces mobara ( Streptoverticillium mobaraense ) into 100ml of transglutaminase fermentation broth produced by adding 1% (w / v) sorbitol, stirring and standing at room temperature for 30min, adding ethanol at a volume ratio of 0.5:1 ethanol:fermentation broth to precipitate the enzyme , high-speed refrigerated centrifuge setting: 6000r / min, 4°C, centrifuge for 20min to obtain a precipitate, put the precipitate into a vacuum drying oven, and dry at 40°C for 3h to obtain a transglutaminase enzyme preparation. Seal the bag with a ziplock, and measure the enzyme activity regularly.