Method for procuding aspergillus oryzae of xylanase capable of efficiently degrading feed substrate and xylanase

[0016] Embodiment 1, separation and screening of strains

[0017] 1. The medium used for separation and screening

[0018] Separation medium: 200g / L peeled potato, 20g / L glucose, 15-20g / L agar, natural pH, sterilized at 121°C for 20min.

[0019] Primary screening medium: add 0.5% (m / v) birch xylan (SigmaX4252-100G) to the separation medium, and sterilize at 121°C for 20 minutes.

[0020] Re-screened solid fermentation medium: bran 70%, corn cob 30%, ammonium sulfate 3%, potassium dihydrogen phosphate 1.5%, (m / m), material-water ratio 1:1-1.2 (m / m). Sterilize at 121°C for 30 minutes.

[0021] 2. Methods and steps of separation and screening

[0022] Mix 1 g of the soil around the bran stack of Zhuhai Feed Factory in Guangdong with 9 mL of sterile water, and after standing for 30 min, take 1 mL of the supernatant as a microbial sample. Take 1mL microbial sample, dilute to 10-3, 10-4, 10-5, 10-6, 10-7, each take 50μl evenly spread on the separation medium (PDA), 30 ℃

[0035] Inoculate the Aspergillus oryzae Y-1 isolated in Example 1 on a fresh wort slope, place it in a constant temperature incubator at 30°C and cultivate it for 84-96h, after the slope is covered with spores, add an appropriate amount of sterile water to wash the spores to prepare into a spore suspension. Put 14g of bran, 6g of corn cob, 0.6g of ammonium sulfate, 0.3g of potassium dihydrogen phosphate, 20ml of water into a 500mL conical flask, and sterilize at 121°C for 30min. After cooling, pick up 1.0mL of Aspergillus oryzae Y1 spore suspension, stir evenly, place in a constant temperature incubator at 30°C and incubate for 72-84h, wait until the surface of the fermentation medium is covered with spores, wash the spores with sterile water, and make a secondary seed Aspergillus oryzae spore suspension.

[0036] Prepare 50kg of dry material fermentation medium (35kg of bran, 15kg of corn cob, 1.5kg of ammonium sulfate, 0.5kg of potassium dihydrogen phosphate), add 50kg of tap

[0039] Inoculate Aspergillus oryzae Y1 on a fresh wort slope, place it in a constant temperature incubator at 30°C and incubate for 84-96 hours. After the slope is covered with spores, add an appropriate amount of sterile water to wash the spores to make the first-grade spores Suspension. Put 14g of bran, 6g of corn cob, 0.6g of ammonium sulfate, 0.3g of potassium dihydrogen phosphate, 20ml of water into a 500mL conical flask, and sterilize at 121°C for 30min. After cooling, pick up 1.0mL of Aspergillus oryzae Y1 spore suspension, stir evenly, place in a constant temperature incubator at 30°C and incubate for 72-84h, wait until the surface of the fermentation medium is covered with spores, wash the spores with sterile water, and make a secondary seed Aspergillus niger spore suspension.

[0040] Prepare 50kg of dry material fermentation medium (35kg of bran, 15kg of corn cob, 1.5kg of ammonium sulfate, 0.5kg of potassium dihydrogen phosphate), add 55kg of tap water, stir well wit