Vomitoxin quantitative determination kit and use method thereof

[0058] The preparation method of the vomitoxin quantitative detection kit of the present invention, the specific steps are as follows:

[0059] 1. Preparation of magnetic separation reagent:

[0060] Select 100 mg of magnetic separation particles (0.1 μm in diameter) with superparamagnetic properties and carboxyl (COOH-) active groups on the surface, and use 0.1 mol / L 2-(N-morpholino)ethanesulfonic acid MES, pH 4.5 solution 10 mL After washing 3 times, the magnetic particles were resuspended in 1 mL of this solution. Then add 50 μL of 10 mg / ml EDC to activate, mix for 2 hours at 25°C, add 2 mg of anti-FITC antibody (goat antibody), and mix for 2 hours at 37°C. Then add an equal volume of 0.01mol / L PBS5%BSA (pH7.4) buffer and block at 37° for 1 hour; finally wash the magnetic beads with 10mL 0.01mol / L PBS (pH7.4) buffer containing 0.5%BSA solution 3 times, and use this solution to make a magnetic separation reagent solution.

[0061] The preparation method of described

[0084] 1. Preparation of magnetic separation reagent:

[0085] Select 100 mg of magnetic separation particles (0.2 μm in diameter) with superparamagnetism and carboxyl (COOH-) active groups on the surface, wash them three times with 10 mL of 0.1 mol / L MES, pH4.5 solution, and rewet the magnetic particles with 1 mL of this solution. hanging. After that, add 75ul of 10mg / ml EDC to activate, mix at 25°C for 2 hours, add 2mg of anti-FITC antibody (mouse anti), and mix at 37°C for 2 hours. Then add an equal volume of 0.01mol / L PBS5%BSA (pH7.4) buffer and block at 37° for 1 hour; finally wash the magnetic beads 3 times with 10mL of 0.01mol / LPBS (pH7.4) buffer containing 0.5% BSA solution , and use this solution to make a magnetic separation reagent solution.

[0086] The preparation method of described MES solution is:

[0087] Weigh MES19.52g, Tris1.56g, NaCl4.24g in a 1L beaker, measure 950mL of purified water to dissolve, adjust the pH to 4.5; add purified water to make u

[0109] 1. Preparation of magnetic separation reagent:

[0110] Select 100 mg of magnetic separation particles (diameter 0.1um) with superparamagnetic properties and carboxyl (COOH-) active groups on the surface, wash with 0.1mol / L MES, pH4.5 solution 10mL for 3 times, and rewet the magnetic particles with 1mL of this solution. hanging. Then add 100ul of 10mg / ml EDC to activate, mix at 25°C for 2 hours, add 2mg of anti-FITC antibody (rabbit anti), and mix at 37°C for 2 hours. Then add an equal volume of 0.01mol / L PBS5%BSA (pH7.4) buffer and block at 37° for 1 hour; finally wash the magnetic beads with 10mL 0.01mol / L PBS (pH7.4) buffer containing 0.5%BSA solution 3 times, and use this solution to make a magnetic separation reagent solution.

[0111] The preparation method of described MES solution is:

[0112] Weigh MES19.52g, Tris1.56g, NaCl4.24g in a 1L beaker, measure 950mL of purified water to dissolve, adjust the pH to 4.5; add purified water to make up to 1L, use a