Preparation and application of mycoplasma pneumoniae recombinant antigen

A technology of mycoplasma pneumoniae and recombinant antigen, which is applied in the field of bioengineering, can solve the problems of increasing the infection probability of experimental operators, cumbersome production process of natural antigen, unfavorable treatment of patients, etc. The method is convenient and fast, suitable for popularization and application, and has good stability Effect

Pending Publication Date: 2020-02-07
南京拂晓生物科技有限公司
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Problems solved by technology

If the detection of IgM antibody is negative, it cannot determine non-mycoplasma pneumoniae infection, and IgG antibody detection is required; at present, the incidence of mycoplasma pneumoniae in my country is on the rise, especially among adolescents, and the current etiological and serological detection methods The operation is complicated and time-consuming, which is not conducive to timely treatment of patients. Among the current serological detection methods, natural antigens are used the most. The producti

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  • Preparation and application of mycoplasma pneumoniae recombinant antigen
  • Preparation and application of mycoplasma pneumoniae recombinant antigen
  • Preparation and application of mycoplasma pneumoniae recombinant antigen

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Embodiment 2

[0037] Application of Recombinant Mycoplasma Pneumoniae Antigen

[0038] (1) Coating protein is high-purity gene recombinant MP protein

[0039] The coating protein is the high-purity gene recombinant MP-1 protein and MP-2 prepared in Example 1.

[0040] (2) Preparation of antigen-coated microtiter plates

[0041] Dilute MP protein 1:10000 with coating buffer (pH value 9.6, 0.1mol / L carbonate buffer), add to the wells of the microplate, 100 μL per well, react at 37°C for 2 hours, shake After removing the coating solution, pat dry, add 200 μL of blocking solution (containing 2% bovine serum albumin phosphate buffer at a final concentration) to each well, react at 37°C for 2 hours, shake off the blocking solution, pat dry, dry, and use Store in aluminum foil bag vacuum packaging.

[0042] 2. Preparation of working concentration enzyme solution

[0043] HRP-labeled goat anti-human IgM needs to be purified by affinity chromatography, with strong species specificity, a purity gr

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Abstract

The invention discloses a technical solution of collecting a sequence of a mycoplasma pneumoniae gene from Genbank, establishing gene data of mycoplasma pneumoniae, and analyzing the gene data throughcomputer software to obtain a segment which has detection activity of mycoplasma pneumoniae adhesion protein P1 (GenBank: AF286371.1) and adhesion protein P30 (GenBank: EF614306.1), and connecting 252nd-355th amino acids (P1A), the 898th-953rd amino acids (P1B), 1274th-1362nd amino acids (P1C), 1589th-1627th amino acids (P1D) of the P1 gene, and a P30 fragment of174th-274th amino acid of the mycoplasma pneumoniae adhesion proteinP30 in series through connecting peptides GSGSGS, wherein the sequence of connections in series is sequentially a P1A fragment-a first connecting peptide-a P1B fragment-a second connecting peptide-P1C-a third connecting peptide-P1C-a fourth connecting peptide-P1D-a fifth connecting peptide-a P30 fragment from a C terminal to an N terminal, and the corresponding nucleic acid sequence is obtained by a chemical synthesis method.

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Claims

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Application Information

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Owner 南京拂晓生物科技有限公司
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