Gold-core-composite nano-carrier as well as preparation method and application thereof

[0055] The preferred gold core composite nanocarrier preparation method of the present invention comprises the following steps:

[0056] (1) The gold nanorod solution modified with hexadecyltrimethylammonium bromide is used as raw material, wherein the length of the gold nanorod is 5-100nm, and the aspect ratio is 1.5-10.0; The rod concentration is 0.1-2mmol / L; the concentration of cetyltrimethylammonium bromide is 0.1-10mmol / L;

[0057] (2) Add sodium hydroxide or ammonia solution to the solution in step (1), make the pH of the solution be 8.0-12.0, then add methanol or ethanol solution of ethyl silicate three times, each interval 10-60min, react in Carrying out at 20-80°C for 1-4 days, wherein the molar ratio of gold nanorods in the gold nanorod aqueous solution to the ethyl silicate is 1:1-50 in terms of gold element and silicon element respectively;

[0058] (3) After the above step (2) is completed, add 3-(methacryloyloxy)propyltrimethoxysilane, the mass of which is 0.0

[0069] The preparation of embodiment 1 gold core composite nano-carrier

[0070] 1) Silica-coated gold nanorods (AuSiO 2 ) preparation

[0071] In a 20mL beaker, mix 7.5mL of an aqueous solution of cetyltrimethylammonium bromide (concentration: 0.1mol / L) with 250 μL of an aqueous solution of chloroauric acid (concentration: 0.01mol / L), add water to The total volume is 9.4mL; then add 0.6mL aqueous sodium borohydride solution (concentration: 0.01mol / L) cooled by ice water; stir vigorously for 3min, place in a 27°C incubator for 3-5h, and obtain a gold nanoparticle seed solution .

[0072]In a 150mL beaker, add 100mL of cetyltrimethylammonium bromide (concentration is 0.1mol / L), 5mL of chloroauric acid aqueous solution (concentration is 0.01mol / L), 0.8mL of silver nitrate aqueous solution (concentration is 0.01mol / L), 2mL sulfuric acid aqueous solution (concentration is 0.5mol / L), and the ascorbic acid aqueous solution (concentration is 0.1mol / L) of 800 μ L; Then

[0079] This example is used to illustrate the reversible process of the particle size of the gold-core composite nanocarrier changing with temperature, and the phase transition temperature of the temperature-sensitive gold-core composite nanocarrier can be judged by the speed of particle size change.

[0080] The gold-core composite nanocarrier prepared in Example 1 was centrifuged and washed once, then 2 mL was taken in a cuvette, and its particle size at different temperatures was measured with a laser particle size analyzer. From 25°C to 35°C, gradually increase the temperature every 5°C, from 35°C to 43°C, gradually increase the temperature every 2°C, from 43°C to 50°C, gradually increase the temperature every 3 or 4°C, equilibrate for 5 minutes at each temperature point, and The particle size of the gold-core composite nanocarriers was measured. Then gradually lower the temperature from 50°C, equilibrate for 5 minutes at each temperature point, and measure the particle size