Method for extracting acetylcholinesterase by use of discarded organ-chicken heads in chicken manufacturing process

[0039] Example 1:

[0040] (1) Weigh the cleaned and dried watch glass, rinse the fresh chicken heads cut vertically with normal saline, use tweezers to take the chicken brain out of the watch glass, remove the non-brain tissue, and weigh it again to calculate the chicken Brain tissue quality m;

[0041] (2) Mix the chicken brain with phosphate buffer (0.1M, pH8.0) containing 1.0% (v / v) TritonX-100 at a ratio of 1:3 (g / mL);

[0042] (3) After homogenizing the mixture obtained in the previous step with a homogenizer for 2 minutes, let it stand for 30 minutes;

[0043] (4) Centrifuge the homogenate at 8000r / min at 4°C for 20min, and take the supernatant to obtain the crude acetylcholinesterase extract. Place the obtained crude extract in a watch glass and perform vacuum freeze-drying to obtain an acetylcholinesterase product. Take 1.0 mg of the acetylcholinesterase product and dissolve it with distilled water to obtain an enzyme solution;

[0044] (5) In a 10mL colorimetric tube, add

[0047] Example 2:

[0048] (1) Weigh the cleaned and dried watch glass, rinse the fresh chicken heads cut vertically with normal saline, use tweezers to take the chicken brain out of the watch glass, remove the non-brain tissue, and weigh it again to calculate the chicken Brain tissue quality m;

[0049] (2) Mix the chicken brain with phosphate buffer (0.1M, pH8.0) containing 1.0% (v / v) TritonX-100 in a ratio of 1:4 (g / mL);

[0050] (3) After homogenizing the mixture obtained in the previous step with a homogenizer for 2 minutes, let it stand for 30 minutes;

[0051] (4) Centrifuge the homogenate at 8000r / min at 4°C for 20min, and take the supernatant to obtain the crude acetylcholinesterase extract. Place the obtained crude extract in a watch glass and perform vacuum freeze-drying to obtain an acetylcholinesterase product. Take 1.0 mg of the acetylcholinesterase product and dissolve it with distilled water to obtain an enzyme solution;

[0052] (5) In a 10 mL colorimetric tube, ad

[0055] Example 3:

[0056] (1) Weigh the cleaned and dried watch glass, rinse the fresh chicken heads cut vertically with normal saline, use tweezers to take the chicken brain out of the watch glass, remove the non-brain tissue, and weigh it again to calculate the chicken Brain tissue quality m;

[0057] (2) Mix chicken brain with phosphate buffer (0.1M, pH8.0) containing 0.5% (v / v) TritonX-100 in a ratio of 1:4 (g / mL);

[0058] (3) After homogenizing the mixture obtained in the previous step with a homogenizer for 2 minutes, let it stand for 30 minutes;

[0059] (4) Centrifuge the homogenate at 8000r / min at 4°C for 20min, and take the supernatant to obtain the crude acetylcholinesterase extract. Place the obtained crude extract in a watch glass and perform vacuum freeze-drying to obtain an acetylcholinesterase product. Take 1.0 mg of the acetylcholinesterase product and dissolve it in distilled water to obtain an enzyme solution;

[0060] (5) Place the supernatant in a watch glass