Method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition

A low-density lipoprotein and catalytic silver technology is applied in the field of double-enzyme synergistic catalytic silver deposition to detect low-density lipoprotein cholesterol. The effect of short detection times

Active Publication Date: 2016-07-20
GUILIN UNIV OF ELECTRONIC TECH
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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for the detection of low-density lipoprotein cholesterol by co-catalyzing silver deposition with

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  • Method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition
  • Method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition
  • Method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition

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Embodiment Construction

[0041] The present invention will be described in detail below in combination with specific embodiments. figure 1 It is a schematic diagram of an electrochemical method for detecting LDL-C based on the principle of dual-enzyme synergistically catalyzed silver deposition reaction. figure 2 Cyclic voltammetry characterization diagrams of different modification processes on the electrode surface; image 3 It is the AC impedance characterization diagram of different modification processes on the electrode surface. The implementation steps are as follows:

[0042] 1. Pretreatment of the electrode: use Al with a particle size of 0.3 μm and 0.05 μm 2 o 3 Grind and polish the surface of the glassy carbon electrode to a mirror surface with the suspension, and then wash the polished electrode with magnetic stirring in pure water and absolute ethanol for 5 minutes, rinse with pure water and then wash with magnetic stirring in pure water for 5 minutes; place the electrode in 30%H in a v

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Abstract

The invention discloses a method for detecting low density lipoprotein cholesterin through double-enzyme concerted catalysis silver deposition. The method comprises the following steps: firstly, forming a sulfydryl-containing poly-aniline phenthiol film on an electrode surface through an electric polymerization method; performing electroreduction on gold ions on the electrode surface through a constant potential deposition method, thereby forming nanometer gold; anchoring the nanometer gold on the electrode surface through sulfydryl on an electric polymer membrane; fixing an apolipoprotein apoB-100 antibody on the nanometer gold; and via identification of the apoB-100 antibody on the specificity of low density lipoprotein, capturing the low density lipoprotein on the electrode surface. Under the synergistic effect of two enzymes of cholesterol esterase and cholesterol oxidase, cholesterol in the low density lipoprotein is decomposed and a weak reducing agent H2O2 is generated; the reducing agent can reduce and deposit silver ions on the gold nanometer grain surface; lastly, a standard curve is drawn by detecting a dissolving volt-ampere current value of an elemental silver, and the detection for the low density lipoprotein cholesterin can be realized.

Description

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Claims

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Application Information

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Owner GUILIN UNIV OF ELECTRONIC TECH
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