Gynogenesis inducing method for palaemon carincauda

A technology for gynogenesis and white shrimp, applied in the field of induction of gynogenesis for white shrimp, achieving simple and effective results

Inactive Publication Date: 2017-07-04
QINGDAO QINGQUAN BIOTECH CO LTD
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Problems solved by technology

But so far, this method has not been reported i

Method used

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Embodiment Construction

[0014] The present invention will be described in further detail below in conjunction with specific embodiments.

[0015] Rearing of parents and collection of sperm and eggs

[0016] White prawns were temporarily raised in circulating seawater at 14°C after being retrieved, and the male and female individuals were kept separately. Feed the bait. During the temporary raising period, the temperature is increased by 1°C every day, and the temperature is kept constant at 16-17°C for production. The eggs were obtained by light stimulation method. After the eggs were produced, the eggs were first filtered through a 300-mesh sieve, and then collected by a 500-mesh sieve. After dilution, the density of the quantitative eggs was observed under a microscope. Adjust the density of eggs according to the experimental needs.

[0017] Electrostimulation Treatment of Eggs of the White Shrimp

[0018] Induction of gynogenesis of eggs: Electric stimulation method is used. Before electric stimu

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Abstract

The invention relates to a gynogenesis inducing method for palaemon carincauda. An electrical stimulation method is adopted; and electrical stimulation treatment is conducted after balancing eggs in an electrical stimulation buffer solution for 2min, regulating parameters of an electrical pulse instrument and sucking 1ml of the eggs into an electric shock cup by virtue of a suction tube, wherein the electrical stimulation buffer solution, which is a mannitol buffer solution, specifically consists of the following ingredients: 0.05mmol/L of CaCl2, 0.1mmol/L of MgCl2, 0.5mmol/L of Hepes, 1% of BSA and 0.27mmol/L of mannitol; and the parameters of the electrical pulse instrument are as follows: field intensity is 1.1kv/cm, capacitance is 3 [mu]F and electrical pulse is conducted twice. During the electrical simulation, an egg density is 4.5 to the power of 4 eggs/ml. The method provided by the invention is simple and effective; and through electrical stimulation inducing, a cleavage rate of the palaemon carincauda can reach 28% or above and an embryo survival rate can reach 98% or above.

Description

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Claims

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Application Information

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Owner QINGDAO QINGQUAN BIOTECH CO LTD
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