Preparation method of composite preparation for preventing and treating poultry respiratory diseases

[0032] Embodiment 1: the extract of Chinese veterinary medicine and powder preparation

[0033] The inventor screened and clinically verified the traditional Chinese veterinary medicines for the treatment of poultry respiratory diseases, and determined that the basic prescription is Yemazhui, and the most preferred ones are Yemazhui, Banlangen, Ephedra, Campanulaceae, Andrographis paniculata, Houttuynia cordata, Astragalus, Tinglizi and Poria cocos The mass ratio is 1 to 10:1:1:1:1:1:1:1:1. The Chinese veterinary medicine extract was prepared by concentrating and extracting the ingredients of traditional Chinese medicine by using ultrasonic technology and vacuum freeze-drying technology.

[0034] After drying Yemachai, pulverize it with a micro pulverizer and mix it, place it in a stoppered conical flask, add 5 times the volume of extraction solvent ethyl acetate, and then use ultrasonic waves to extract its active ingredients. The soaking time before extraction is 60- 90min.

[0038] Embodiment 2: the development of chicken egg yolk immunoglobulin powder

[0039] Select healthy laying hens, and use commercial or self-made monovalent or multivalent vaccines for respiratory pathogenic microorganisms (such as Newcastle disease-infectious bronchitis-avian influenza triple inactivated vaccine or Newcastle disease-infectious bursa-infectious bronchitis triple vaccine) Vaccine) Inject multi-point injections into the breast muscles of laying hens, 0.5mL / hen, and immunize once a week. After three immunizations, the immunoglobulin level reaches a high titer, and the eggs are collected.

[0040] Extract yolk immunoglobulin (taking the acetic acid-sodium acetate buffer method as an example), the eggs are first washed with water to remove the dirt on the surface of the eggshell, immersed in 0.5% bromogeramine solution, soaked for 10 minutes, and then rinsed with 75% alcohol, Aseptically break the eggshell, pour it into a sterilized container, separate the egg y

[0043] Example 3: Optimum ratio selection of Chinese veterinary drug egg yolk immunoglobulin compound preparation

[0044] According to different ratios, the optimal ratio of Chinese veterinary medicine and chicken egg yolk immunoglobulin was studied.

[0045] According to a completely random design, 200 5-week-old unimmunized AA broiler roosters were randomly divided into 4 test groups and 1 control group according to body weight, with 40 chickens in each group. According to the different proportions (mass ratio) of the Chinese veterinary medicine extract powder described in Example 1 and the egg yolk immunoglobulin described in Example 2, they were divided into test groups I, II, III and IV, and the proportions were 1:1 respectively. , 1:0.8, 1:0.5, 1:0.2. Each chicken in the test group was fed with 1.5ml of the aqueous solution of the Chinese veterinary drug egg yolk immunoglobulin compound preparation every day, and the control group was fed with 1.5ml of normal saline as