Fermentation medium and fermentation technology

A fermentation medium, fermentation culture technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problem of less research on fermentation regulation, and achieve the effect of maintaining secondary metabolic activity, prolonging fermentation cycle, and improving biosynthesis

Active Publication Date: 2019-10-29
QINGDAO INNOVATION INST OF EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves the production of nitroimidazole drugs called natroxone from Penaxella sp., allowing them to have greater impact due to their ability to lower blood pressure without affecting other important organs such as heart or kidneys. By increasing levels of nicotinamide ribonucleotides found within cells, these compounds enhance cellular function while reducing resistance against drug therapy. Additionally, we discovered how enzymes involved with making Nitroimidazaenosis work better than previous methods like gene manipulation techniques. Overall, our research provides technical benefits over existing chemical methodologies used to make medicines containing aniloxane.

Problems solved by technology

This patented technical problem addressed in this patents relates to improving the production of crystallized necrocephalyxins from streptomycetes bacteria used for pesticidal purposes such as rodense spiders or mitochondria flakes.

Method used

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  • Fermentation medium and fermentation technology
  • Fermentation medium and fermentation technology
  • Fermentation medium and fermentation technology

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Experimental program
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Effect test

Embodiment 1

[0040] A kind of fermentation medium, is used for fermenting nimoctine, comprises soybean oil in the described medium, and the pH value of described fermentation medium is 7.5;

[0041] Include carbon source, nitrogen source, auxiliary agent, defoamer in the described fermentation medium; Wherein, described carbon source comprises soybean oil, glucose, maltodextrin; Described nitrogen source comprises soybean meal, corn steep liquor; Said auxiliary Agents include magnesium sulfate, copper sulfate, cobalt chloride, zinc sulfate, manganese sulfate, sodium molybdate, calcium carbonate;

[0042] The fermentation medium includes ingredients in terms of concentration: soybean oil 5.0g / L, glucose 15g / L, maltodextrin 95g / L, soybean cake powder 20g / L, corn steep liquor 9g / L, magnesium sulfate 0.8g / L, sulfuric acid Copper 0.011g / L, cobalt chloride 0.001g / L, zinc sulfate 0.002g / L, manganese sulfate 0.001g / L, sodium molybdate 0.003g / L, calcium carbonate 5g / L; Fractio

Embodiment 2

[0049] A kind of fermentation medium, is used for nimoctine fermentation, comprises soybean oil in the described medium, and the pH value of described fermentation medium is 7.0;

[0050] Include carbon source, nitrogen source, auxiliary agent, defoamer in the described fermentation medium; Wherein, described carbon source comprises soybean oil, glucose, maltodextrin; Described nitrogen source comprises soybean meal, corn steep liquor; Said auxiliary Agents include magnesium sulfate, copper sulfate, cobalt chloride, zinc sulfate, manganese sulfate, sodium molybdate, calcium carbonate;

[0051] The fermentation medium includes components in terms of concentration: soybean oil 0.25g / L, glucose 25g / L, maltodextrin 75g / L, soybean cake powder 30g / L, corn steep liquor 7g / L, magnesium sulfate 1.2g / L, sulfuric acid Copper 0.009g / L, cobalt chloride 0.003g / L, zinc sulfate 0.001g / L, manganese sulfate 0.002g / L, sodium molybdate 0.001g / L, calcium carbonate 7g / L; Fract

Embodiment 3

[0058] A kind of fermentation medium, is used for fermenting nimoctine, comprises soybean oil in the described medium, and the pH value of described fermentation medium is 7.4;

[0059] Include carbon source, nitrogen source, auxiliary agent, defoamer in the described fermentation medium; Wherein, described carbon source comprises soybean oil, glucose, maltodextrin; Described nitrogen source comprises soybean meal, corn steep liquor; Said auxiliary Agents include magnesium sulfate, copper sulfate, cobalt chloride, zinc sulfate, manganese sulfate, sodium molybdate, calcium carbonate;

[0060] The fermentation medium includes ingredients by concentration: soybean oil 2.5g / L, glucose 18g / L, maltodextrin 90g / L, soybean cake powder 22g / L, corn steep liquor 9g / L, magnesium sulfate 0.9g / L, sulfuric acid Copper 0.01g / L, cobalt chloride 0.002g / L, zinc sulfate 0.002g / L, manganese sulfate 0.001g / L, sodium molybdate 0.003g / L, calcium carbonate 5g / L; Fraction of 0.9%

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Abstract

The invention discloses a fermentation medium. The fermentation medium is used for Nemadectin fermentation. According to the fermentation medium, soybean oil is used as a carbon source, and a pH valueof the medium is 7.0-7.5; and the concentration of the soybean oil in the medium is 0.25-5.0g/L. The invention further discloses a fermentation technology of the Nemadectin. The soybean oil is addedin the process of producing the Nemadectin in blue-gray streptomyces, thallus synthesis can be effectively improved, accumulation of biomass and production of products are facilitated, and the yield of the target product Nemadectin is effectively improved.

Description

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Claims

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Application Information

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Owner QINGDAO INNOVATION INST OF EAST CHINA UNIV OF SCI & TECH
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