Fipronil and methoprene dihydric alcohol plastid, and preparation method and application thereof

[0031] Another aspect of the present invention, proposes the preparation method of fiprenil and methoprene glycol plastosome, comprises the following steps:

[0032] (1) Take feprenil, methoprene, dipalmitophosphatidylcholine, glycol (ethanol: propylene glycol = 6.5: 3.5) and sodium lauryl sulfate according to the formula described in any of the above , sealed and stirred until dissolved;

[0033] (2) Stir while adding phosphate buffer solution dropwise, and cool to room temperature;

[0034] (3) Ultrasound to obtain nano-alcoholsomes;

[0035] (4) Fiprenil and methoprene diol plastom was obtained by filtration.

[0036] Preferably, the stirring temperature in step (1) is 29.5-30.5°C, the stirring speed is 600-800rpm; the dropping speed in step (2) is 3-6mL / min; the pH of the phosphate buffer is 6.5-7.0; the stirring time is 20-30min .

[0037] Preferably, step (3) ultrasonic power 100~120W, frequency 45~50kHz; ultrasonic 3~4s, interval 3~4s, 30~40 cycles; Step (4) 50~100nm

[0043] A kind of fiprenil and methoprene glycol plastosome, comprising following parts by weight: 5.0 parts of fiprenil; 4.0 parts of methoprene; 2.0 parts of dipalmitone phosphatidylcholine; glycol ( Ethanol:propylene glycol=6.5:3.5) 30.0 parts; sodium lauryl sulfate, 0.5 parts and 55 parts of phosphate buffer (pH=6.5). (take the total amount of 100ml as an example)

[0044] A kind of preparation method of fiprenyl and methoprene glycol plastid:

[0045] (1) Take by weighing fiprenil, methoprene, dipalmitophosphatidylcholine, glycol (ethanol: propylene glycol=6.5:3.5), sodium lauryl sulfate and add reaction tank by proportioning, stirring temperature is 30°C, stir at 700rpm, stir until dissolved;

[0046](2) Then add phosphate buffer (pH=6.5) at a drop rate of 3mL / min, stir in an airtight container for 20 minutes until completely dissolved, and cool to room temperature;

[0047] (3) Under ultrasonic power 100W and ultrasonic frequency 45kHz, ultrasonic 3s, interval 3s, carr

[0051] A kind of fiprenil and methoprene glycol plastosome, comprising following components by weight: 6.0 parts of fiprenil; 5.0 parts of methoprene; 2.5 parts of dipalmitone phosphatidylcholine; glycol ( Ethanol: propylene glycol=6.5:3.5) 35.0 parts; sodium lauryl sulfate 0.5 part and 70 parts of phosphate buffer (pH=6.5). (take the total amount of 100ml as an example)

[0052] A kind of preparation method of fiprenyl and methoprene glycol plastid:

[0053] (1) Take by weighing fiprenil, methoprene, dipalmitophosphatidylcholine, glycol (ethanol: propylene glycol=6.5:3.5), sodium lauryl sulfate and add reaction tank by proportioning, stirring temperature is 30°C, stir at 700rpm, stir until dissolved;

[0054] (2) Then add phosphate buffer (pH=6.5) at a drop rate of 3mL / min, stir in an airtight container for 20 minutes until completely dissolved, and cool to room temperature;

[0055] (3) Under ultrasonic power 100W and ultrasonic frequency 45kHz, ultrasonic 3s, interval 3s,