Substrate covered with polymers for selective separation of cells or for cell culturing

A polymer and matrix technology, applied in cell culture support/coating, tissue cell/virus culture device, separation of microorganisms, etc., can solve the problems of damage ligand cell selectivity, insufficient immobilization, etc., and achieve excellent Excellent cell separation ability, excellent adhesion, and excellent separation ability

Pending Publication Date: 2020-03-24
UNIVERSITY OF TOYAMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology describes how certain molecules can attach themselves onto surfaces like plastics without causing them stick together again when they come off their original position on these materials. These specialized structures preventing unwanted interactions between different types of material from sticking out over time. They also allow other substances (like drugs) attached thereto to easily move around freely within this structure. Overall, it provides an effective way to culture living organisms by controllably attaching small particles called “ligatures” into surface features made up mostly of plasma membranes.

Problems solved by technology

This patent describes various techniques related to separating biological particles such as red blood corpuscles, white blood cells, tumour tissue cells, bone marrow stem cells, brain glial cells, cancer metastasis suppressive agents, enzymes involved in drug development processes, and gene therapy vectors. These technologies aim to improve efficiency and effectiveness during these procedures while reducing damage caused by unwanted components present within the body's own cells.

Method used

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  • Substrate covered with polymers for selective separation of cells or for cell culturing
  • Substrate covered with polymers for selective separation of cells or for cell culturing
  • Substrate covered with polymers for selective separation of cells or for cell culturing

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Experimental program
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Embodiment

[0218] Hereinafter, although an Example is given and this invention is demonstrated more concretely, this invention is not limited to the following Example.

Synthetic example 1

[0219] Synthesis Example 1 Synthesis of Polymers with Zwitterions and Click Reaction Sites

[0220] 3.0 g of CMB (N-methacryloyloxyethyl-N,N-dimethylammonium-α-N-methylcarboxybetaine, manufactured by Osaka Organic Chemical Industry Co., Ltd.), PGMA (methacrylic acid propargyl ester) 4.0 g, and 2,2'-azobis[N-(2-carboxyethyl)-2-methylpropionamidine] hydrate (VA-057, manufactured by Wako Pure Chemical Industries, Ltd. ) 0.07 g was dissolved in 28.4 g of ethyl lactate, and then heated and stirred at 80° C. overnight under a nitrogen atmosphere. Then, ethanol was added to the reaction solution to precipitate and recover the polymer, and the polymer was dried under reduced pressure to obtain a polymer having a structure represented by the following formula (1). The weight average molecular weight of the obtained polymer was 8,000 in terms of standard polyethylene oxide and polyethylene glycol.

[0221] [chemical formula 25]

[0222]

Synthetic example 2

[0223] Synthesis Example 2 Synthesis of Polymers with Zwitterions, Click Reaction Sites, and Silane Coupling Groups

[0224] A terpolymer called poly(CMB-PGMA-MPTMS) was synthesized, which used methacryloxypropyltrimethoxysilane (MPTMS) which can modify the surface of glass and metal with chemical bonds. It is currently known that when the composition ratio of MPTMS is 10 mol%, the coverage rate is the best, and can inhibit the adsorption and adhesion of proteins and cells caused by CMB (J.Biomed.Mater.Res.A, 2016, 104A, 2029 -2036.), so the composition ratio of MPTMS was set as 10 mol%, and the composition ratios of CMB and PGMA were 80:10, 70:20, and 60:30 terpolymers. Dissolve a total of 45mmol of CMB and PGMA, 0.5mmol of MPTMS, and 0.17mmol of AIBN in a mixed solvent of ethanol / dimethylformamide (3:1), react at 70°C for 4 hours under a nitrogen atmosphere, and then supplement By adding 0.034 mmol of AIBN and further reacting for 4 hours, a polymer having a structure repr

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Abstract

A liganded substrate having at least part of the surface thereof covered by a polymer (P1) containing a structural unit represented by formula (1a) and (1b), a raw material of said liganded substrate,and a production method thereof are provided. (In the formula, R1, R2, X, Y, L, Q1, Q2, Q3, m1, m2 and n are as set forth in the claims and description.

Description

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Claims

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Application Information

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Owner UNIVERSITY OF TOYAMA
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